Beyond Genes: Inclusion of Alternative Splicing and Alternative Polyadenylation to Assess the Genetic Architecture of Predisposition to Voluntary Alcohol Consumption in Brain of the HXB/BXH Recombinant Inbred Rat Panel.
HXB/BXH recombinant inbred panel
RNA-seq—RNA sequencing
alternative polyadenylation
alternative splicing
isoform
transcriptome
voluntary alcohol consumption
weighted gene co expression network analysis
Journal
Frontiers in genetics
ISSN: 1664-8021
Titre abrégé: Front Genet
Pays: Switzerland
ID NLM: 101560621
Informations de publication
Date de publication:
2022
2022
Historique:
received:
23
11
2021
accepted:
10
02
2022
entrez:
4
4
2022
pubmed:
5
4
2022
medline:
5
4
2022
Statut:
epublish
Résumé
Post transcriptional modifications of RNA are powerful mechanisms by which eukaryotes expand their genetic diversity. For instance, researchers estimate that most transcripts in humans undergo alternative splicing and alternative polyadenylation. These splicing events produce distinct RNA molecules, which in turn yield distinct protein isoforms and/or influence RNA stability, translation, nuclear export, and RNA/protein cellular localization. Due to their pervasiveness and impact, we hypothesized that alternative splicing and alternative polyadenylation in brain can contribute to a predisposition for voluntary alcohol consumption. Using the HXB/BXH recombinant inbred rat panel (a subset of the Hybrid Rat Diversity Panel), we generated over one terabyte of brain RNA sequencing data (total RNA) and identified novel splice variants (
Identifiants
pubmed: 35368676
doi: 10.3389/fgene.2022.821026
pii: 821026
pmc: PMC8965255
doi:
Types de publication
Journal Article
Langues
eng
Pagination
821026Subventions
Organisme : NIDA NIH HHS
ID : P30 DA044223
Pays : United States
Organisme : NIAAA NIH HHS
ID : R24 AA013162
Pays : United States
Informations de copyright
Copyright © 2022 Lusk, Hoffman, Mahaffey, Rosean, Smith, Silhavy, Pravenec, Tabakoff and Saba.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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