Innate Lymphoid Cells Are Required to Induce Airway Hyperreactivity in a Murine Neutrophilic Asthma Model.
airway hyperreactivity
innate lymphoid cells (ILCs)
murine model
neutrophilic inflammation
non-allergic asthma
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2022
2022
Historique:
received:
05
01
2022
accepted:
14
02
2022
entrez:
4
4
2022
pubmed:
5
4
2022
medline:
9
4
2022
Statut:
epublish
Résumé
Non-allergic asthma is driven by multiple endotypes of which neutrophilic and pauci-granulocytic asthma have been best established. However, it is still puzzling what drives inflammation and airway hyperreactivity (AHR) in these patients and how it can be treated effectively. Recently, a potential role of the innate immune system and especially the innate lymphoid cells (ILC) has been proposed. In this study, we investigated the effects of LPS inhalation on airway inflammation and AHR as a potential model for elucidating the pathogenesis of non-allergic asthma. Wild-type (BALB/c), SCID, IL-17A In this study, we developed a murine model for non-allergic neutrophilic asthma. We found that repeated endonasal applications of low-dose LPS in BALB/c mice led to AHR, BAL neutrophilia, and a significant increase in lung ILC3 as well as a significant increase in lung chemokines KC and MIP-2 and cytokines IL-1β, IL-17A, IL-22, and TNF. The adoptive transfer of ILC in Rag2 In conclusion, we report here a murine model for neutrophilic asthma where ILC are required to induce airway hyperreactivity.
Identifiants
pubmed: 35371094
doi: 10.3389/fimmu.2022.849155
pmc: PMC8965562
doi:
Substances chimiques
Cytokines
0
Interleukin-17
0
Lipopolysaccharides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
849155Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2022 Jonckheere, Seys, Steelant, Decaesteker, Dekoster, Cremer, Dilissen, Schols, Iwakura, Vande Velde, Breynaert, Schrijvers, Vanoirbeek, Ceuppens, Dupont and Bullens.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Am J Respir Crit Care Med. 2017 Aug 1;196(3):283-297
pubmed: 28252317
Eur Respir J. 2019 Nov 7;54(5):
pubmed: 31439682
Am J Respir Crit Care Med. 2014 May 15;189(10):1284-5
pubmed: 24832749
Nat Immunol. 2015 Jan;16(1):45-56
pubmed: 25521684
Eur Respir J. 2016 Aug;48(2):380-92
pubmed: 27126687
Allergy Asthma Proc. 2019 May 1;40(3):138-145
pubmed: 31018888
Eur Respir J. 2009 Jul;34(1):200-8
pubmed: 19251789
Nature. 2015 Jan 15;517(7534):293-301
pubmed: 25592534
Methods. 2001 Dec;25(4):386-401
pubmed: 11846608
Respir Res. 2021 May 22;22(1):158
pubmed: 34022896
Am J Physiol Lung Cell Mol Physiol. 2008 Sep;295(3):L379-99
pubmed: 18621912
Front Immunol. 2020 Dec 18;11:613170
pubmed: 33391283
J Allergy Clin Immunol. 2020 Mar;145(3):808-817.e2
pubmed: 31805312
Sci Rep. 2019 Nov 26;9(1):17598
pubmed: 31772203
Respir Med. 2005 Apr;99(4):429-43
pubmed: 15763449
Curr Opin Immunol. 2018 Oct;54:102-108
pubmed: 29986301
Cell. 2018 Aug 23;174(5):1054-1066
pubmed: 30142344
Curr Allergy Asthma Rep. 2019 Nov 28;19(12):59
pubmed: 31781873
J Asthma. 2017 Jun;54(5):447-455
pubmed: 27589490
Clin Exp Allergy. 2013 Sep;43(9):1009-17
pubmed: 23957336
Int J Mol Sci. 2020 Jan 23;21(3):
pubmed: 31979396
Respir Res. 2017 Feb 23;18(1):39
pubmed: 28231834
BMC Pulm Med. 2020 Jan 10;20(1):9
pubmed: 31924190
Cytokine Growth Factor Rev. 2011 Aug;22(4):189-95
pubmed: 22019906
Nat Med. 2009 Apr;15(4):410-6
pubmed: 19330007
Eur J Pharmacol. 2001 Nov 23;431(3):365-71
pubmed: 11730731
Am J Physiol Lung Cell Mol Physiol. 2015 Aug 1;309(3):L271-9
pubmed: 26024893
J Allergy Clin Immunol. 2020 Jun;145(6):1499-1509
pubmed: 32507228
CSH Protoc. 2008 May 01;2008:pdb.prot4986
pubmed: 21356829
Arch Med Res. 2017 Nov;48(8):717-726
pubmed: 29224909
Nat Med. 2012 May 04;18(5):716-25
pubmed: 22561835
Immun Inflamm Dis. 2020 Sep;8(3):393-407
pubmed: 32567222
Sci Rep. 2020 Sep 30;10(1):16181
pubmed: 32999350
Front Immunol. 2021 Mar 18;12:635522
pubmed: 33868259
J Clin Med. 2017 Nov 29;6(12):
pubmed: 29186064
Biol Pharm Bull. 2020;43(1):31-35
pubmed: 31902928
Am J Respir Crit Care Med. 2013 Mar 1;187(5):486-93
pubmed: 23262517
PLoS One. 2012;7(2):e32110
pubmed: 22355412
Respir Res. 2017 Jun 19;18(1):123
pubmed: 28629359
Am J Respir Cell Mol Biol. 2010 Jan;42(1):96-104
pubmed: 19346316
Cell Rep. 2019 Dec 24;29(13):4509-4524.e5
pubmed: 31875557
Methods Mol Biol. 2017;1559:121-136
pubmed: 28063042
Am J Respir Crit Care Med. 2000 Dec;162(6):2295-301
pubmed: 11112154
PLoS One. 2013 Jun 21;8(6):e67115
pubmed: 23805294
Sci Rep. 2021 Apr 8;11(1):7777
pubmed: 33833346
Sci Rep. 2016 Aug 16;6:31663
pubmed: 27526865
Science. 2015 Sep 4;349(6252):1106-10
pubmed: 26339029
Exp Ther Med. 2018 Mar;15(3):2492-2498
pubmed: 29456654
Am J Respir Cell Mol Biol. 2013 Mar;48(3):382-9
pubmed: 23239494
Respir Med. 2003 Jun;97(6):726-33
pubmed: 12814161
Curr Opin Allergy Clin Immunol. 2019 Feb;19(1):53-60
pubmed: 30516548
J Allergy Clin Immunol. 2018 Apr;141(4):1280-1290
pubmed: 28756296
Lancet. 2018 Jan 27;391(10118):350-400
pubmed: 28911920
Nat Med. 2012 Mar 04;18(4):547-54
pubmed: 22388091
Clin Respir J. 2008 Oct;2 Suppl 1:88-91
pubmed: 20298356
Exp Mol Med. 2020 Jan;52(1):92-104
pubmed: 31956268
Immun Inflamm Dis. 2022 Feb;10(2):123-129
pubmed: 34889061
Nat Med. 2014 Jan;20(1):54-61
pubmed: 24336249
J Allergy Clin Immunol. 2013 Mar;131(3):636-45
pubmed: 23360759
J Allergy Clin Immunol. 2015 Feb;135(2):379-85
pubmed: 25195169
Eur Respir J. 2014 Apr;43(4):1067-76
pubmed: 24136334
J Allergy Clin Immunol. 2020 Feb;145(2):496-498
pubmed: 31812572
Am J Respir Crit Care Med. 2015 Sep 15;192(6):660-8
pubmed: 26161792