A Role of Caveolae in Trabecular Meshwork Mechanosensing and Contractile Tone.
Rho/ROCK pathway
caveolae
caveolin-1 (CAV1)
caveolin-1 scaffolding domain peptide
contractility and relaxation
cyclic stretch
primary open angle glaucoma
trabecular meshwork
Journal
Frontiers in cell and developmental biology
ISSN: 2296-634X
Titre abrégé: Front Cell Dev Biol
Pays: Switzerland
ID NLM: 101630250
Informations de publication
Date de publication:
2022
2022
Historique:
received:
14
01
2022
accepted:
03
03
2022
entrez:
4
4
2022
pubmed:
5
4
2022
medline:
5
4
2022
Statut:
epublish
Résumé
Polymorphisms in the CAV1/2 gene loci impart increased risk for primary open-angle glaucoma (POAG). CAV1 encodes caveolin-1 (Cav1), which is required for biosynthesis of plasma membrane invaginations called caveolae. Cav1 knockout mice exhibit elevated intraocular pressure (IOP) and decreased outflow facility, but the mechanistic role of Cav1 in IOP homeostasis is unknown. We hypothesized that caveolae sequester/inhibit RhoA, to regulate trabecular meshwork (TM) mechanosensing and contractile tone. Using phosphorylated myosin light chain (pMLC) as a surrogate indicator for Rho/ROCK activity and contractile tone, we found that pMLC was elevated in Cav1-deficient TM cells compared to control (131 ± 10%,
Identifiants
pubmed: 35372369
doi: 10.3389/fcell.2022.855097
pii: 855097
pmc: PMC8969750
doi:
Types de publication
Journal Article
Langues
eng
Pagination
855097Subventions
Organisme : NEI NIH HHS
ID : P30 EY005722
Pays : United States
Organisme : NEI NIH HHS
ID : P30 EY021725
Pays : United States
Organisme : NEI NIH HHS
ID : R01 EY022359
Pays : United States
Informations de copyright
Copyright © 2022 De Ieso, Kuhn, Bernatchez, Elliott and Stamer.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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