From Macroscopy to Ultrastructure: An Integrative Approach to Pulmonary Pathology.

histology imaging lung lung disease microCT

Journal

Frontiers in medicine
ISSN: 2296-858X
Titre abrégé: Front Med (Lausanne)
Pays: Switzerland
ID NLM: 101648047

Informations de publication

Date de publication:
2022
Historique:
received: 21 01 2022
accepted: 07 02 2022
entrez: 4 4 2022
pubmed: 5 4 2022
medline: 5 4 2022
Statut: epublish

Résumé

Pathology and radiology are complimentary tools, and their joint application is often crucial in obtaining an accurate diagnosis in non-neoplastic pulmonary diseases. However, both come with significant limitations of their own: Computed Tomography (CT) can only visualize larger structures due to its inherent-relatively-poor resolution, while (histo) pathology is often limited due to small sample size and sampling error and only allows for a 2D investigation. An innovative approach of inflating whole lung specimens and subjecting these subsequently to CT and whole lung microCT allows for an accurate matching of CT-imaging and histopathology data of exactly the same areas. Systematic application of this approach allows for a more targeted assessment of localized disease extent and more specifically can be used to investigate early mechanisms of lung diseases on a morphological and molecular level. Therefore, this technique is suitable to selectively investigate changes in the large and small airways, as well as the pulmonary arteries, veins and capillaries in relation to the disease extent in the same lung specimen. In this perspective we provide an overview of the different strategies that are currently being used, as well as how this growing field could further evolve.

Identifiants

DOI: 10.3389/fmed.2022.859337 PMID: 35372395 PMC: PMC8965844
pubmed: 35372395
doi: 10.3389/fmed.2022.859337
pmc: PMC8965844
doi:

Types de publication

Journal Article

Langues

eng

Pagination

859337

Informations de copyright

Copyright © 2022 Verleden, Braubach, Werlein, Plucinski, Kuhnel, Snoeckx, El Addouli, Welte, Haverich, Laenger, Dettmer, Pauwels, Verplancke, Van Schil, Lapperre, Kwakkel-Van-Erp, Ackermann, Hendriks and Jonigk.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Stijn E Verleden (SE)

Antwerp Surgical Training, Anatomy and Research Centre (ASTARC), Antwerp University, Antwerp, Belgium.
Division of Pneumology, University Hospital Antwerp, Edegem, Belgium.
Department of Thoracic and Vascular Surgery, University Hospital Antwerp, Edegem, Belgium.

Peter Braubach (P)

Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany.
Institute for Pathology, Hannover Medical School, Hannover, Germany.

Christopher Werlein (C)

Institute for Pathology, Hannover Medical School, Hannover, Germany.

Edith Plucinski (E)

Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany.
Institute for Pathology, Hannover Medical School, Hannover, Germany.

Mark P Kuhnel (MP)

Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany.
Institute for Pathology, Hannover Medical School, Hannover, Germany.

Annemiek Snoeckx (A)

Division of Radiology, University Hospital Antwerp and University of Antwerp, Edegem, Belgium.

Haroun El Addouli (H)

Division of Radiology, University Hospital Antwerp and University of Antwerp, Edegem, Belgium.

Tobias Welte (T)

Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany.
Division of Pneumology, Hannover Medical School, Hannover, Germany.

Axel Haverich (A)

Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany.
Division of Thoracic Surgery, Hannover Medical School, Hannover, Germany.

Florian P Laenger (FP)

Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany.
Institute for Pathology, Hannover Medical School, Hannover, Germany.

Sabine Dettmer (S)

Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany.
Department of Radiology, Hannover Medical School, Hannover, Germany.

Patrick Pauwels (P)

Division of Pathology, University Hospital Antwerp, Edegem, Belgium.

Veronique Verplancke (V)

Division of Pneumology, University Hospital Antwerp, Edegem, Belgium.

Paul E Van Schil (PE)

Antwerp Surgical Training, Anatomy and Research Centre (ASTARC), Antwerp University, Antwerp, Belgium.
Department of Thoracic and Vascular Surgery, University Hospital Antwerp, Edegem, Belgium.

Therese Lapperre (T)

Division of Pneumology, University Hospital Antwerp, Edegem, Belgium.
Laboratory of Experimental Medicine and Pediatrics (LEMP), Antwerp University, Antwerp, Belgium.

Johanna M Kwakkel-Van-Erp (JM)

Division of Pneumology, University Hospital Antwerp, Edegem, Belgium.
Laboratory of Experimental Medicine and Pediatrics (LEMP), Antwerp University, Antwerp, Belgium.

Maximilian Ackermann (M)

Institute of Pathology and Department of Molecular Pathology, Helios University Clinic Wuppertal, University of Witten-Herdecke, Witten, Germany.
Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany.

Jeroen M H Hendriks (JMH)

Antwerp Surgical Training, Anatomy and Research Centre (ASTARC), Antwerp University, Antwerp, Belgium.
Department of Thoracic and Vascular Surgery, University Hospital Antwerp, Edegem, Belgium.

Danny Jonigk (D)

Member of the German Center for Lung Research (DZL), Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Hannover, Germany.
Institute for Pathology, Hannover Medical School, Hannover, Germany.

Classifications MeSH