Sas20 is a highly flexible starch-binding protein in the Ruminococcus bromii cell-surface amylosome.
Ruminococcus bromii
amylosome
carbohydrate-binding module
resistant starch
Journal
The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R
Informations de publication
Date de publication:
05 2022
05 2022
Historique:
received:
26
01
2022
revised:
22
03
2022
accepted:
24
03
2022
pubmed:
5
4
2022
medline:
7
6
2022
entrez:
4
4
2022
Statut:
ppublish
Résumé
Ruminococcus bromii is a keystone species in the human gut that has the rare ability to degrade dietary resistant starch (RS). This bacterium secretes a suite of starch-active proteins that work together within larger complexes called amylosomes that allow R. bromii to bind and degrade RS. Starch adherence system protein 20 (Sas20) is one of the more abundant proteins assembled within amylosomes, but little could be predicted about its molecular features based on amino acid sequence. Here, we performed a structure-function analysis of Sas20 and determined that it features two discrete starch-binding domains separated by a flexible linker. We show that Sas20 domain 1 contains an N-terminal β-sandwich followed by a cluster of α-helices, and the nonreducing end of maltooligosaccharides can be captured between these structural features. Furthermore, the crystal structure of a close homolog of Sas20 domain 2 revealed a unique bilobed starch-binding groove that targets the helical α1,4-linked glycan chains found in amorphous regions of amylopectin and crystalline regions of amylose. Affinity PAGE and isothermal titration calorimetry demonstrated that both domains bind maltoheptaose and soluble starch with relatively high affinity (K
Identifiants
pubmed: 35378131
pii: S0021-9258(22)00336-2
doi: 10.1016/j.jbc.2022.101896
pmc: PMC9112005
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Carrier Proteins
0
Dietary Carbohydrates
0
Starch
9005-25-8
Amylose
9005-82-7
Amylopectin
9037-22-3
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101896Subventions
Organisme : NIGMS NIH HHS
ID : P30 GM138395
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM103622
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest The authors declare that they have no conflicts of interest with the contents of this article.
Références
Environ Microbiol. 2018 Jan;20(1):324-336
pubmed: 29159997
Nucleic Acids Res. 2018 Jul 2;46(W1):W363-W367
pubmed: 29860391
J Struct Biol. 2021 Sep;213(3):107765
pubmed: 34186214
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
Mol Microbiol. 2015 Jan;95(2):209-30
pubmed: 25388295
Methods Enzymol. 2012;510:211-31
pubmed: 22608728
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42
pubmed: 21460441
Nature. 2017 Jan 19;541(7637):407-411
pubmed: 28077872
Structure. 2008 Jul;16(7):1105-15
pubmed: 18611383
Microbiome. 2016 Jun 29;4(1):33
pubmed: 27357127
Methods Mol Biol. 2017;1588:119-127
pubmed: 28417364
Crit Rev Biotechnol. 2016;36(3):578-84
pubmed: 25582732
J Mol Biol. 1993 Jan 5;229(1):105-24
pubmed: 7678431
Curr Opin Struct Biol. 2016 Oct;40:33-42
pubmed: 27450115
Nat Protoc. 2015 Jun;10(6):845-58
pubmed: 25950237
Proc Natl Acad Sci U S A. 1999 Apr 13;96(8):4246-51
pubmed: 10200247
Appl Microbiol Biotechnol. 2010 Feb;85(5):1241-9
pubmed: 19908036
Adv Nutr. 2013 Nov 06;4(6):587-601
pubmed: 24228189
BMC Genomics. 2012 May 30;13:210
pubmed: 22646801
J Biol Chem. 2001 Nov 16;276(46):43010-7
pubmed: 11560933
ISME J. 2012 Aug;6(8):1535-43
pubmed: 22343308
Front Microbiol. 2018 Nov 05;9:2558
pubmed: 30455672
Cell Mol Life Sci. 2019 Nov;76(21):4319-4340
pubmed: 31062073
Nat Rev Microbiol. 2017 Feb;15(2):83-95
pubmed: 27941816
Cell Mol Life Sci. 2016 Jul;73(14):2603-17
pubmed: 27137179
J Mol Biol. 2000 Sep 8;302(1):205-17
pubmed: 10964570
mBio. 2015 Sep 29;6(5):e01058-15
pubmed: 26419877
Nucleic Acids Res. 2016 Jan 4;44(D1):D7-19
pubmed: 26615191
Biotechnol Biofuels. 2017 Sep 18;10:222
pubmed: 28932263
Methods Enzymol. 1997;276:307-26
pubmed: 27754618
J Appl Crystallogr. 2017 Sep 05;50(Pt 5):1545-1553
pubmed: 29021737
Arch Biochem Biophys. 2000 Jul 15;379(2):237-44
pubmed: 10898940
Proteins. 1997 Dec;29(4):517-27
pubmed: 9408948
Cell Mol Life Sci. 2016 Jul;73(14):2619-41
pubmed: 27137180
J Biol Chem. 2006 Jan 6;281(1):587-98
pubmed: 16230347
Trends Microbiol. 2020 Feb;28(2):95-108
pubmed: 31624005
J Struct Biol. 2014 Nov;188(2):188-93
pubmed: 25270376
J Mol Biol. 2005 May 20;348(5):1211-24
pubmed: 15854656
Biotechnol Adv. 2019 Dec;37(8):107451
pubmed: 31536775
J Biol Chem. 2002 Oct 25;277(43):40887-92
pubmed: 12186865
Proteins. 2009 Aug 1;76(2):516-9
pubmed: 19382205
DNA Seq. 2001 Jul;12(1):27-37
pubmed: 11697143
Biotechnol Biofuels. 2015 Aug 13;8:114
pubmed: 26269713
Curr Opin Biotechnol. 2020 Feb;61:66-71
pubmed: 31765963
Curr Opin Gastroenterol. 2015 Jan;31(1):69-75
pubmed: 25394236
Protein Sci. 2019 Feb;28(2):454-463
pubmed: 30371978
J Mol Biol. 1988 May 20;201(2):365-78
pubmed: 3418703
J Biol Chem. 2005 Nov 18;280(46):38562-8
pubmed: 16157599
Mol Biol Evol. 2001 May;18(5):691-9
pubmed: 11319253
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Nucleic Acids Res. 2015 Jul 1;43(W1):W389-94
pubmed: 25883141
J Biol Chem. 2018 Mar 16;293(11):4201-4212
pubmed: 29367338
J Mol Recognit. 2005 Nov-Dec;18(6):491-501
pubmed: 16167300
PLoS One. 2010 Nov 29;5(11):e15046
pubmed: 21151493
Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):861-877
pubmed: 31588918
Biochem J. 2004 Sep 15;382(Pt 3):769-81
pubmed: 15214846
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
FEBS Lett. 1995 Feb 27;360(2):121-4
pubmed: 7875315
Nature. 2013 Apr 25;496(7446):477-81
pubmed: 23619693
Genome Med. 2018 Aug 1;10(1):62
pubmed: 30068376
Nucleic Acids Res. 2012 Jul;40(Web Server issue):W445-51
pubmed: 22645317
Environ Microbiol. 2015 Sep;17(9):3407-26
pubmed: 25845888
Nature. 2015 Jan 8;517(7533):165-169
pubmed: 25567280
Evolution. 1985 Jul;39(4):783-791
pubmed: 28561359
Cell. 2016 Jun 2;165(6):1332-1345
pubmed: 27259147
Nucleic Acids Res. 2022 Jan 7;50(D1):D543-D552
pubmed: 34723319
Enzyme Microb Technol. 2017 Feb;97:43-54
pubmed: 28010772
Protein Sci. 2020 Jan;29(1):66-75
pubmed: 31576635
Biophys J. 2005 Apr;88(4):2823-32
pubmed: 15653742
Mol Biol Evol. 2020 Apr 1;37(4):1237-1239
pubmed: 31904846
Physiol Rev. 2010 Jul;90(3):859-904
pubmed: 20664075
Nat Commun. 2015 Jun 26;6:7481
pubmed: 26112186
World J Gastroenterol. 2011 Mar 28;17(12):1519-28
pubmed: 21472114
J Biol Chem. 2012 Oct 5;287(41):34614-25
pubmed: 22910908
Gene. 1997 Oct 1;198(1-2):149-57
pubmed: 9370276
Acta Crystallogr D Struct Biol. 2016 Dec 1;72(Pt 12):1254-1266
pubmed: 27917826
Int J Biol Macromol. 1998 Aug;23(2):85-112
pubmed: 9730163
Annu Rev Plant Biol. 2010;61:209-34
pubmed: 20192737
Nucleic Acids Res. 2016 Jul 8;44(W1):W424-9
pubmed: 27151198
Mol Microbiol. 2018 Jan;107(2):249-264
pubmed: 29139580
Sci Rep. 2018 May 8;8(1):7204
pubmed: 29739979
Nucleic Acids Res. 2014 Jul;42(Web Server issue):W320-4
pubmed: 24753421
Nucleic Acids Res. 2014 Jan;42(Database issue):D490-5
pubmed: 24270786