Anisotropic presentation of ligands on cargos modulates degradative function of phagosomes.
Journal
Biophysical reports
ISSN: 2667-0747
Titre abrégé: Biophys Rep (N Y)
Pays: United States
ID NLM: 9918266001106676
Informations de publication
Date de publication:
09 Mar 2022
09 Mar 2022
Historique:
entrez:
6
4
2022
pubmed:
7
4
2022
medline:
7
4
2022
Statut:
ppublish
Résumé
Anisotropic arrangement of cell wall components is ubiquitous among bacteria and fungi, but how such functional anisotropy affects interactions between microbes and host immune cells is not known. Here we address this question with regard to phagosome maturation, the process used by host immune cells to degrade internalized microbes. We developed two-faced microparticles as model pathogens that display ligands on only one hemisphere and simultaneously function as fluorogenic sensors for probing biochemical reactions inside phagosomes during degradation. The fluorescent indicator on just one hemisphere gives the particle sensors a moon-like appearance. We show that anisotropic presentation of ligands on particles delays the start of acidification and proteolysis in phagosomes, but does not affect their degradative capacity. Our work suggests that the spatial presentation of ligands on pathogens plays a critical role in modulating the degradation process in phagosomes during host-pathogen interactions.
Identifiants
pubmed: 35382229
doi: 10.1016/j.bpr.2021.100041
pmc: PMC8978551
mid: NIHMS1787434
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM124918
Pays : United States
Références
Science. 2002 Dec 6;298(5600):1942-6
pubmed: 12471245
J Leukoc Biol. 2013 Oct;94(4):657-70
pubmed: 23610146
Biol Chem Hoppe Seyler. 1992 Jul;373(7):433-40
pubmed: 1515071
Bioeng Transl Med. 2017 Jan 19;2(1):92-101
pubmed: 29313025
Nat Cell Biol. 2015 Jul;17(7):893-906
pubmed: 26098576
Proc Natl Acad Sci U S A. 2011 Oct 18;108(42):17396-401
pubmed: 21969579
Langmuir. 2015 Mar 10;31(9):2833-8
pubmed: 25674706
Nature. 2011 Apr 28;472(7344):471-5
pubmed: 21525931
J Cell Biol. 2018 Jan 2;217(1):329-346
pubmed: 29089378
Nat Methods. 2012 Jun 10;9(7):724-6
pubmed: 22688415
Proc Natl Acad Sci U S A. 1993 Apr 1;90(7):3033-7
pubmed: 8464919
Microbiol Mol Biol Rev. 2006 Sep;70(3):660-703
pubmed: 16959965
J Am Chem Soc. 2013 Dec 26;135(51):19091-4
pubmed: 24308498
Nanoscale. 2017 Jan 07;9(1):288-297
pubmed: 27909711
Biophys J. 2018 Jun 19;114(12):2900-2909
pubmed: 29925026
PLoS One. 2013 Apr 22;8(4):e60989
pubmed: 23630577
Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14556-61
pubmed: 22908282
J Cell Biol. 2005 Apr 11;169(1):139-49
pubmed: 15809313
Langmuir. 2008 May 6;24(9):4944-51
pubmed: 18355095
Cell. 2016 Jan 14;164(1-2):128-140
pubmed: 26771488
Cell Microbiol. 2008 Oct;10(10):2058-66
pubmed: 18549457
Methods Mol Biol. 2008;445:311-25
pubmed: 18425459
J Leukoc Biol. 1998 May;63(5):521-33
pubmed: 9581795
J Cell Biol. 2001 Oct 1;155(1):19-25
pubmed: 11581283
Biophys J. 2013 May 7;104(9):1886-92
pubmed: 23663831
J Cell Biol. 2010 Nov 29;191(5):999-1012
pubmed: 21115805
Annu Rev Pathol. 2012;7:61-98
pubmed: 21910624
J Leukoc Biol. 2013 Jun;93(6):921-32
pubmed: 23543768
Science. 2004 May 14;304(5673):1014-8
pubmed: 15143282
Nat Rev Mol Cell Biol. 2008 Aug;9(8):639-49
pubmed: 18612320
Antibiotics (Basel). 2020 May 07;9(5):
pubmed: 32392749
Nat Cell Biol. 2007 Apr;9(4):367-78
pubmed: 17351642
Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):4930-4
pubmed: 16549762
Biophys J. 2012 Aug 8;103(3):395-404
pubmed: 22947855
Nanoscale. 2014 Jun 21;6(12):6837-43
pubmed: 24827972
PLoS One. 2010 Apr 06;5(4):e10051
pubmed: 20386614
Biochem J. 2002 Sep 15;366(Pt 3):689-704
pubmed: 12061891
J Biol Chem. 1990 Dec 5;265(34):21099-107
pubmed: 2147429
Nat Rev Microbiol. 2009 Apr;7(4):274-86
pubmed: 19287450
Nat Rev Immunol. 2009 Aug;9(8):594-600
pubmed: 19590530
Appl Microbiol Biotechnol. 2005 Aug;68(2):163-73
pubmed: 15818477
FEMS Microbiol Rev. 2021 Mar 16;45(2):
pubmed: 32970796
Proc Natl Acad Sci U S A. 2010 Nov 9;107(45):19332-7
pubmed: 20974965
Curr Biol. 2001 Oct 16;11(20):1631-5
pubmed: 11676926
Mol Cell. 1998 Jul;2(1):157-62
pubmed: 9702203
Nat Rev Mol Cell Biol. 2008 Oct;9(10):781-95
pubmed: 18813294
FEMS Microbiol Rev. 2016 Jul;40(4):480-93
pubmed: 27075488
Proc Natl Acad Sci U S A. 2015 Apr 14;112(15):4636-41
pubmed: 25825728