Introduction of quorum sensing elements into bacterial bioreporter circuits enhances explosives' detection capabilities.
2,4,6‐trinitrotoluene (TNT)
2,4‐dinitrotoluene (DNT)
bioluminescence
bioreporter
biosensor
explosives
quorum sensing
Journal
Engineering in life sciences
ISSN: 1618-0240
Titre abrégé: Eng Life Sci
Pays: Germany
ID NLM: 101193313
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
17
10
2021
revised:
13
01
2022
accepted:
11
02
2022
entrez:
6
4
2022
pubmed:
7
4
2022
medline:
7
4
2022
Statut:
epublish
Résumé
A possible solution for the standoff detection of buried landmines is based on the use of microbial bioreporters, genetically engineered to emit a remotely detectable optical signal in response to trace amounts of explosives' signature chemicals, mostly 2,4-dinitrotoluene (DNT). Previously developed DNT sensor strains were based on the fusion of a DNT-inducible gene promoter to a reporting element, either a fluorescent protein gene or a bacterial bioluminescence gene cassette. In the present study, a different approach was used: the DNT-inducible promoter activates, in
Identifiants
pubmed: 35382532
doi: 10.1002/elsc.202100134
pii: ELSC1479
pmc: PMC8961053
doi:
Types de publication
Journal Article
Langues
eng
Pagination
308-318Informations de copyright
© 2022 The Authors. Engineering in Life Sciences published by Wiley‐VCH GmbH.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Nat Biotechnol. 2017 Apr 11;35(4):308-310
pubmed: 28398330
Science. 1997 Sep 5;277(5331):1453-62
pubmed: 9278503
Annu Rev Microbiol. 2001;55:165-99
pubmed: 11544353
Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9505-9
pubmed: 8790360
Nature. 2004 Apr 22;428(6985):868-71
pubmed: 15064770
N Biotechnol. 2020 Nov 25;59:65-73
pubmed: 32622861
Front Microbiol. 2017 Jun 16;8:1097
pubmed: 28670299
Appl Microbiol Biotechnol. 2015 Sep;99(17):7177-88
pubmed: 25981994
Mikrobiologiia. 2006 Jul-Aug;75(4):457-64
pubmed: 17025169
Front Microbiol. 2016 Sep 22;7:1490
pubmed: 27713734
Mol Microbiol. 2012 Jun;84(5):795-806
pubmed: 22500943
Environ Microbiol. 2020 Mar;22(3):801-807
pubmed: 32011091
Annu Rev Genet. 2001;35:439-68
pubmed: 11700290
Appl Microbiol Biotechnol. 2021 May;105(10):4329-4337
pubmed: 33942130
Appl Microbiol Biotechnol. 2014 Jan;98(2):885-95
pubmed: 23615740
Biosens Bioelectron. 2016 May 15;79:784-8
pubmed: 26774094
Mol Syst Biol. 2006;2:2006.0008
pubmed: 16738554
Methods Mol Biol. 1998;102:247-58
pubmed: 9680625
Talanta. 2001 May 10;54(3):501-13
pubmed: 18968274
J Am Soc Mass Spectrom. 2008 Oct;19(10):1425-34
pubmed: 18672381
J Bacteriol. 1987 Nov;169(11):5101-12
pubmed: 2822665
Microb Biotechnol. 2021 Jan;14(1):251-261
pubmed: 33095504
Microb Biotechnol. 2010 May;3(3):300-10
pubmed: 21255329
Front Bioeng Biotechnol. 2022 Feb 14;10:821835
pubmed: 35237579