Epigenetic reprogramming in cloned mouse embryos following treatment with DNA methyltransferase and histone deacetylase inhibitors.
DNA methylation
RG108
Somatic cell nuclear transfer
histone acetylation
suberoylanilide hydroxamic acid (SAHA)
Journal
Systems biology in reproductive medicine
ISSN: 1939-6376
Titre abrégé: Syst Biol Reprod Med
Pays: England
ID NLM: 101464963
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
pubmed:
7
4
2022
medline:
10
6
2022
entrez:
6
4
2022
Statut:
ppublish
Résumé
We examined the effects of DNA methyltransferase inhibitor - RG108, and histone deacetylase inhibitor - SAHA, on the reprogramming parameters of cloned mouse embryos produced by somatic cell nuclear transfer into oocytes. The programming parameters studied included dynamics of histone reacetylation, developmental rate, DNA methylation, and transcript levels of genes, all of which are pivotal to lineage specification and blastocyst formation. At the pronuclear stage, somatic nucleus-transplanted oocytes treated with 5 µM SAHA presented higher histone acetylation at H3K9, H3K14, H4K16 and H4K12, compared to untreated clones (
Identifiants
pubmed: 35382652
doi: 10.1080/19396368.2022.2036868
doi:
Substances chimiques
Histone Deacetylase Inhibitors
0
Histones
0
DNA
9007-49-2
Methyltransferases
EC 2.1.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM