Western blotting of native proteins from agarose gels.

Blotting, Western COVID-19 Electrophoresis, Agar Gel / methods Electrophoresis, Polyacrylamide Gel Gels Humans Proteins / chemistry SARS-CoV-2 Sepharose / chemistry Spike Glycoprotein, Coronavirus
GFP PLXDC2 SARS-CoV-2 spike protein agarose conformation specific monoclonal antibody native gel electrophoresis western blotting

Journal

BioTechniques
ISSN: 1940-9818
Titre abrégé: Biotechniques
Pays: England
ID NLM: 8306785

Informations de publication

Date de publication:
05 2022
Historique:
pubmed: 7 4 2022
medline: 24 5 2022
entrez: 6 4 2022
Statut: ppublish

Résumé

We have developed a new Western blotting method of native proteins from agarose-based gel electrophoresis using a buffer at pH 6.1 containing basic histidine and acidic 2-(

Identifiants

DOI: 10.2144/btn-2022-0012 PMID: 35383476
pubmed: 35383476
doi: 10.2144/btn-2022-0012
doi:

Substances chimiques

Gels 0
Proteins 0
Spike Glycoprotein, Coronavirus 0
spike protein, SARS-CoV-2 0
Sepharose 9012-36-6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

207-218

Auteurs

Chiaki Sakuma (C)

Research & Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-shi, Ibaraki, 318-0004, Japan.

Masataka Nakagawa (M)

Research & Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-shi, Ibaraki, 318-0004, Japan.

Yui Tomioka (Y)

Research & Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-shi, Ibaraki, 318-0004, Japan.

Toshiaki Maruyama (T)

Abwiz Bio Inc., 9823 Pacific Heights Blvd, suite J, San Diego, CA 9212, USA.

Kevin Entzminger (K)

Abwiz Bio Inc., 9823 Pacific Heights Blvd, suite J, San Diego, CA 9212, USA.

Jonathan K Fleming (JK)

Abwiz Bio Inc., 9823 Pacific Heights Blvd, suite J, San Diego, CA 9212, USA.

Takashi Shibata (T)

Research & Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-shi, Ibaraki, 318-0004, Japan.

Yasunori Kurosawa (Y)

Research & Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-shi, Ibaraki, 318-0004, Japan.
Abwiz Bio Inc., 9823 Pacific Heights Blvd, suite J, San Diego, CA 9212, USA.

C J Okumura (CJ)

Abwiz Bio Inc., 9823 Pacific Heights Blvd, suite J, San Diego, CA 9212, USA.

Tsutomu Arakawa (T)

Alliance Protein Laboratories, 13380 Pantera Rd, San Diego, CA 92130, USA.

Teruo Akuta (T)

Research & Development Division, Kyokuto Pharmaceutical Industrial Co., Ltd., 3333-26, Aza-Asayama, Kamitezuna Takahagi-shi, Ibaraki, 318-0004, Japan.
ORCID: 0000-0002-0669-4379

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Dosage immunologique › Immunotransfert › Technique de Western › Western blotting of native proteins from agarose gels.
questionsmedicales.fr › Maladies de l'appareil respiratoire › Infections de l'appareil respiratoire › Pneumopathie infectieuse › Pneumopathie virale › COVID-19 › Western blotting of native proteins from agarose gels.
questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Électrophorèse › Électrophorèse sur gel d'agar › Western blotting of native proteins from agarose gels.
questionsmedicales.fr › Techniques d'investigation › Techniques électrochimiques › Électrophorèse › Électrophorèse sur gel de polyacrylamide › Western blotting of native proteins from agarose gels.
questionsmedicales.fr › Préparations pharmaceutiques › Colloïdes › Gels › Western blotting of native proteins from agarose gels.
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Western blotting of native proteins from agarose gels.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Western blotting of native proteins from agarose gels.
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Nidovirales › Coronaviridae › Coronavirus › Betacoronavirus › Virus du SRAS › SARS-CoV-2 › Western blotting of native proteins from agarose gels.
questionsmedicales.fr › Glucides › Polyosides › Agarose › Western blotting of native proteins from agarose gels.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales structurales › Protéines de l'enveloppe virale › Protéines de fusion virale › Glycoprotéine de spicule des coronavirus › Western blotting of native proteins from agarose gels.