Proteomics as a tool to gain next level insights into photo-crosslinkable biopolymer modifications.

(Photo-crosslinkable) biomaterials Biomaterial characterization Chemical modifications Localization of functionalizations Proteomics

Journal

Bioactive materials
ISSN: 2452-199X
Titre abrégé: Bioact Mater
Pays: China
ID NLM: 101685294

Informations de publication

Date de publication:
Nov 2022
Historique:
received: 05 10 2021
revised: 04 01 2022
accepted: 16 01 2022
entrez: 7 4 2022
pubmed: 8 4 2022
medline: 8 4 2022
Statut: epublish

Résumé

The distribution of photo-crosslinkable moieties onto a protein backbone can affect a biomaterial's crosslinking behavior, and therefore also its mechanical and biological properties. A profound insight in this respect is essential for biomaterials exploited in tissue engineering and regenerative medicine. In the present work, photo-crosslinkable moieties have been introduced on the primary amine groups of: (i) a recombinant collagen peptide (RCPhC1) with a known amino acid (AA) sequence, and (ii) bovine skin collagen (COL BS) with an unknown AA sequence. The degree of substitution (DS) was quantified with two conventional techniques: an

Identifiants

pubmed: 35386456
doi: 10.1016/j.bioactmat.2022.01.023
pii: S2452-199X(22)00031-7
pmc: PMC8965084
doi:

Types de publication

Journal Article

Langues

eng

Pagination

204-220

Informations de copyright

© 2022 The Authors.

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Auteurs

Nele Pien (N)

Ghent University, Polymer Chemistry and Biomaterials Research Group, Centre of Macromolecular Chemistry (CMaC), Krijgslaan 281 S4bis, 9000 Gent, Belgium.
Laval University, Laboratory for Biomaterials and Bioengineering, CRC-I, Pavillon Pouliot, Québec G1V 0A6, Canada.

Fabrice Bray (F)

Univ. Lille, CNRS, USR 3290, MSAP, Miniaturisation pour la Synthèse l'Analyse et la Protéomique, F-59 000, Lille, France.

Tom Gheysens (T)

Ghent University, Polymer Chemistry and Biomaterials Research Group, Centre of Macromolecular Chemistry (CMaC), Krijgslaan 281 S4bis, 9000 Gent, Belgium.

Liesbeth Tytgat (L)

Ghent University, Polymer Chemistry and Biomaterials Research Group, Centre of Macromolecular Chemistry (CMaC), Krijgslaan 281 S4bis, 9000 Gent, Belgium.

Christian Rolando (C)

Univ. Lille, CNRS, USR 3290, MSAP, Miniaturisation pour la Synthèse l'Analyse et la Protéomique, F-59 000, Lille, France.

Diego Mantovani (D)

Laval University, Laboratory for Biomaterials and Bioengineering, CRC-I, Pavillon Pouliot, Québec G1V 0A6, Canada.

Peter Dubruel (P)

Ghent University, Polymer Chemistry and Biomaterials Research Group, Centre of Macromolecular Chemistry (CMaC), Krijgslaan 281 S4bis, 9000 Gent, Belgium.

Sandra Van Vlierberghe (SV)

Ghent University, Polymer Chemistry and Biomaterials Research Group, Centre of Macromolecular Chemistry (CMaC), Krijgslaan 281 S4bis, 9000 Gent, Belgium.

Classifications MeSH