Proteomics Profiling to Distinguish DOCK8 Deficiency From Atopic Dermatitis.

atopic dermatitis biomarker dedicator of cytokinesis (DOCK8) hyper IgE syndrome (HIES) label-free proteomics multiple reaction monitoring

Journal

Frontiers in allergy
ISSN: 2673-6101
Titre abrégé: Front Allergy
Pays: Switzerland
ID NLM: 9918227355906676

Informations de publication

Date de publication:
2021
Historique:
received: 13 09 2021
accepted: 22 10 2021
entrez: 7 4 2022
pubmed: 8 4 2022
medline: 8 4 2022
Statut: epublish

Résumé

Dedicator of cytokinesis 8 deficiency is an autosomal recessive primary immune deficiency disease belonging to the group of hyperimmunoglobulinemia E syndrome (HIES). The clinical phenotype of dedicator of cytokinesis 8 (DOCK8) deficiency, characterized by allergic manifestations, increased infections, and increased IgE levels, overlaps with the clinical presentation of atopic dermatitis (AD). Despite the identification of metabolomics and cytokine biomarkers, distinguishing between the two conditions remains clinically challenging. The present study used a label-free untargeted proteomics approach using liquid-chromatography mass spectrometry with network pathway analysis to identify the differentially regulated serum proteins and the associated metabolic pathways altered between the groups. Serum samples from DOCK8 (

Identifiants

pubmed: 35386989
doi: 10.3389/falgy.2021.774902
pmc: PMC8974780
doi:

Types de publication

Journal Article

Langues

eng

Pagination

774902

Informations de copyright

Copyright © 2021 Jacob, Masood, Shinwari, Abdel Jabbar, Al-Mousa, Arnaout, AlSaud, Dasouki, Alaiya and Abdel Rahman.

Déclaration de conflit d'intérêts

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Auteurs

Minnie Jacob (M)

Metabolomics Section, Department of Clinical Genomics, Center for Genomics Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.

Afshan Masood (A)

Proteomics Resource Unit, Obesity Research Center, College of Medicine, King Saud University, Riyadh, Saudi Arabia.

Zakiya Shinwari (Z)

Proteomics Unit, Stem-Cell and Tissue Re-engineering Program, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.

Mai Abdel Jabbar (M)

Metabolomics Section, Department of Clinical Genomics, Center for Genomics Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.

Hamoud Al-Mousa (H)

Section of Pediatric Allergy and Immunology, Department of Pediatrics, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.

Rand Arnaout (R)

Section of Pediatric Allergy and Immunology, Department of Pediatrics, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.

Bandar AlSaud (B)

Section of Pediatric Allergy and Immunology, Department of Pediatrics, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.

Majed Dasouki (M)

Metabolomics Section, Department of Clinical Genomics, Center for Genomics Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.

Ayodele A Alaiya (AA)

Proteomics Unit, Stem-Cell and Tissue Re-engineering Program, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.

Anas M Abdel Rahman (AM)

Metabolomics Section, Department of Clinical Genomics, Center for Genomics Medicine, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
Department of Biochemistry and Molecular Medicine, College of Medicine, Alfaisal University, Riyadh, Saudi Arabia.
Department of Chemistry, Memorial University of Newfoundland, St. John's, NL, Canada.

Classifications MeSH