Polyelectrolyte Multilayers in Capillary Electrophoresis.

electro-osmotic flow electrophoresis polyelectrolyte multilayers proteins separation efficiency

Journal

ChemPlusChem
ISSN: 2192-6506
Titre abrégé: Chempluschem
Pays: Germany
ID NLM: 101580948

Informations de publication

Date de publication:
28 Mar 2022
Historique:
revised: 19 03 2022
received: 31 01 2022
entrez: 7 4 2022
pubmed: 8 4 2022
medline: 8 4 2022
Statut: aheadofprint

Résumé

Capillary electrophoresis (CE) has been proven to be a performant analytical method to analyze both small and macro molecules. Indeed, it is capable of separating compounds of the same nature according to differences in their charge to size ratios, particularly proteins, monoclonal antibodies and peptides. However, one of the major obstacles to reach high separation efficiency remains the adsorption of solutes on the capillary wall. Among the different coating approaches used to control and minimize solute adsorption, polyelectrolyte multilayers can be applied to CE as a versatile approach. These coatings are made up of alternating layers of polycations and polyanions, and may be used in acidic, neutral or basic conditions depending on the solutes to be analyzed. This Review provides an overview of Successive Multiple Ionic-polymer Layer (SMIL) coatings used in CE, looking at how different parameters induce variations on the electro-osmotic flow (EOF), separation efficiency and coating stability, as well as their promising applications in the biopharmaceutical field.

Identifiants

pubmed: 35388990
doi: 10.1002/cplu.202200028
doi:

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202200028

Subventions

Organisme : ANR-PRCI
ID : ANR-DEF-2020-NLE

Informations de copyright

© 2022 Wiley-VCH GmbH.

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Auteurs

Sébastien Roca (S)

IBMM, University of Montpellier, CNRS, ENSCM, Montpellier, France.

Laura Dhellemmes (L)

IBMM, University of Montpellier, CNRS, ENSCM, Montpellier, France.

Laurent Leclercq (L)

IBMM, University of Montpellier, CNRS, ENSCM, Montpellier, France.

Hervé Cottet (H)

IBMM, University of Montpellier, CNRS, ENSCM, Montpellier, France.

Classifications MeSH