Ionic Combisomes: A New Class of Biomimetic Vesicles to Fuse with Life.
amphiphilic comb polymers
bottom-up synthetic biology
hybrid vesicles
polyelectrolyte-surfactant complexes
polymersomes
synthetic biomembranes
vesicle fusion
Journal
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
ISSN: 2198-3844
Titre abrégé: Adv Sci (Weinh)
Pays: Germany
ID NLM: 101664569
Informations de publication
Date de publication:
06 2022
06 2022
Historique:
revised:
03
03
2022
received:
31
01
2022
pubmed:
9
4
2022
medline:
15
6
2022
entrez:
8
4
2022
Statut:
ppublish
Résumé
The construction of biomembranes that faithfully capture the properties and dynamic functions of cell membranes remains a challenge in the development of synthetic cells and their application. Here a new concept for synthetic cell membranes based on the self-assembly of amphiphilic comb polymers into vesicles, termed ionic combisomes (i-combisomes) is introduced. These combs consist of a polyzwitterionic backbone to which hydrophobic tails are linked by electrostatic interactions. Using a range of microscopies and molecular simulations, the self-assembly of a library of combs in water is screened. It is discovered that the hydrophobic tails form the membrane's core and force the backbone into a rod conformation with nematic-like ordering confined to the interface with water. This particular organization resulted in membranes that combine the stability of classic polymersomes with the biomimetic thickness, flexibility, and lateral mobility of liposomes. Such unparalleled matching of biophysical properties and the ability to locally reconfigure the molecular topology of its constituents enable the harboring of functional components of natural membranes and fusion with living bacteria to "hijack" their periphery. This provides an almost inexhaustible palette to design the chemical and biological makeup of the i-combisomes membrane resulting in a powerful platform for fundamental studies and technological applications.
Identifiants
pubmed: 35393756
doi: 10.1002/advs.202200617
pmc: PMC9189634
doi:
Substances chimiques
Liposomes
0
Polymers
0
Water
059QF0KO0R
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2200617Subventions
Organisme : European Commission within a H2020-NMBP-TR-IND -2018, EVPRO
ID : 814495-2
Organisme : Deutsche Forschungsgemeinschaft
ID : SFB 985
Organisme : Russian Science Foundation
ID : 22-43-04417
Organisme : Russian Foundation for Basic Research
ID : 20-53-12023
Informations de copyright
© 2022 The Authors. Advanced Science published by Wiley-VCH GmbH.
Références
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2022 Mar;14(2):e1761
pubmed: 34725945
Proc Natl Acad Sci U S A. 2020 Jun 30;117(26):15006-15017
pubmed: 32554497
Angew Chem Int Ed Engl. 2018 Oct 8;57(41):13382-13392
pubmed: 29749673
Adv Mater. 2015 Apr 17;27(15):2459-65
pubmed: 25732045
Soft Matter. 2017 Jan 18;13(3):627-637
pubmed: 27991638
Angew Chem Int Ed Engl. 2017 Sep 4;56(37):10998-11002
pubmed: 28678351
Langmuir. 2017 Feb 21;33(7):1705-1715
pubmed: 28128560
Biomacromolecules. 2019 Feb 11;20(2):712-727
pubmed: 30354069
Langmuir. 2011 Apr 19;27(8):4884-90
pubmed: 21405067
Nature. 1978 Feb 16;271(5646):672-4
pubmed: 625336
J Phys D Appl Phys. 2018 Aug;51(34):
pubmed: 30655651
Nano Lett. 2005 Dec;5(12):2343-9
pubmed: 16351175
Acc Chem Res. 2017 Apr 18;50(4):769-777
pubmed: 28094501
Soft Matter. 2021 Jan 22;17(2):254-267
pubmed: 32789415
Science. 2004 Sep 3;305(5689):1474-6
pubmed: 15353806
Adv Biosyst. 2019 Jun;3(6):e1800324
pubmed: 32648708
Angew Chem Int Ed Engl. 2015 Mar 23;54(13):4036-40
pubmed: 25656452
Proc Natl Acad Sci U S A. 2019 Jul 30;116(31):15378-15385
pubmed: 31308223
Chem Rev. 2016 Feb 24;116(4):2023-78
pubmed: 26583535
Annu Rev Biomed Eng. 2006;8:323-41
pubmed: 16834559
Proc Natl Acad Sci U S A. 2016 Mar 1;113(9):E1134-41
pubmed: 26884210
J Am Chem Soc. 2013 Jun 19;135(24):9055-77
pubmed: 23692629
Adv Biosyst. 2019 May;3(5):e1800330
pubmed: 32627412
Langmuir. 2016 May 17;32(19):4896-903
pubmed: 27142706
Langmuir. 2004 Feb 3;20(3):540-3
pubmed: 15773070
Science. 1999 May 14;284(5417):1143-6
pubmed: 10325219
ACS Nano. 2011 Mar 22;5(3):1775-84
pubmed: 21344879
Science. 2010 May 21;328(5981):1009-14
pubmed: 20489021
Faraday Discuss. 2013;161:305-31; discussion 419-59
pubmed: 23805747
J Am Chem Soc. 2002 Oct 9;124(40):11872-3
pubmed: 12358530
Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1162-7
pubmed: 26787853
Sci Adv. 2016 Apr 15;2(4):e1500948
pubmed: 27152331
Proc Natl Acad Sci U S A. 2020 Sep 8;117(36):21896-21905
pubmed: 32843347
Nat Struct Mol Biol. 2008 Jul;15(7):675-83
pubmed: 18596814
Biochim Biophys Acta. 1987 Oct 5;906(3):309-52
pubmed: 3307918
Adv Colloid Interface Sci. 2014 Jun;208:189-96
pubmed: 24529971
Chemistry. 2017 Jul 12;23(39):9391-9396
pubmed: 28513997
Biophys J. 1986 Jun;49(6):1155-70
pubmed: 3719074
Eur Phys J E Soft Matter. 2011 Feb;34(2):14
pubmed: 21337017
J Am Chem Soc. 2011 Dec 21;133(50):20507-20
pubmed: 22066981
Biophys J. 2000 Jul;79(1):321-7
pubmed: 10866958
Proc Natl Acad Sci U S A. 2019 Mar 19;116(12):5376-5382
pubmed: 30819900
Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5202-6
pubmed: 22431595
Nano Lett. 2019 Aug 14;19(8):5732-5738
pubmed: 31306030
J Am Chem Soc. 2009 Apr 22;131(15):5705-13
pubmed: 19323552
Science. 2002 Aug 9;297(5583):967-73
pubmed: 12169723
Proc Natl Acad Sci U S A. 2019 Feb 19;116(8):2837-2842
pubmed: 30718416
Adv Sci (Weinh). 2022 Jun;9(17):e2200617
pubmed: 35393756
Cell. 2004 Jan 23;116(2):153-66
pubmed: 14744428
Biophys J. 2000 Jul;79(1):328-39
pubmed: 10866959
Angew Chem Int Ed Engl. 2017 Jun 1;56(23):6515-6518
pubmed: 28444913
Nano Lett. 2015 Jun 10;15(6):3871-8
pubmed: 26013972
Nano Lett. 2017 Nov 8;17(11):6816-6821
pubmed: 29067800
Biochemistry. 1989 May 2;28(9):3692-703
pubmed: 2751990
Angew Chem Int Ed Engl. 2021 Apr 6;60(15):8352-8360
pubmed: 33493389
Eur Biophys J. 1989;17(2):101-11
pubmed: 2766997
Macromol Rapid Commun. 2022 Mar;43(5):e2100712
pubmed: 34820929
J Phys Chem B. 2010 Dec 16;114(49):16625-31
pubmed: 21086974
Proc Natl Acad Sci U S A. 2019 Jan 15;116(3):744-752
pubmed: 30591566
Anat Rec. 2002 Nov 1;268(3):208-14
pubmed: 12382319
Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9058-63
pubmed: 24927561
Trends Biotechnol. 2018 Sep;36(9):938-951
pubmed: 29685820
Proc Natl Acad Sci U S A. 2020 Jun 2;117(22):11931-11939
pubmed: 32424105
Langmuir. 2007 Jan 30;23(3):1307-14
pubmed: 17241051
Angew Chem Int Ed Engl. 2012 Nov 12;51(46):11616-9
pubmed: 23055422
Biophys J. 1984 Feb;45(2):399-420
pubmed: 6365189
Nat Commun. 2021 Aug 17;12(1):4972
pubmed: 34404795
Chem Soc Rev. 2018 Nov 26;47(23):8572-8610
pubmed: 30177983
J Am Chem Soc. 2011 Mar 9;133(9):2881-3
pubmed: 21322595
Nat Cell Biol. 2007 Jan;9(1):7-14
pubmed: 17199125
J Phys Chem B. 2008 Nov 20;112(46):14655-64
pubmed: 18729503
Chem Soc Rev. 2016 Jan 21;45(2):377-411
pubmed: 26563574
Biophys J. 2000 Jan;78(1):290-305
pubmed: 10620293