Longitudinal dynamics of the bovine udder microbiota.
Bovine milk
Microbiota
Temporal dynamics
Journal
Animal microbiome
ISSN: 2524-4671
Titre abrégé: Anim Microbiome
Pays: England
ID NLM: 101759457
Informations de publication
Date de publication:
08 Apr 2022
08 Apr 2022
Historique:
received:
22
10
2021
accepted:
30
03
2022
entrez:
9
4
2022
pubmed:
10
4
2022
medline:
10
4
2022
Statut:
epublish
Résumé
In recent years, the number of studies concerning microbiota of the intramammary environment has increased rapidly due to the development of high-throughput sequencing technologies that allow mapping of microbiota without culturing. This has revealed that an environment previously thought to be sterile in fact harbours a microbial community. Since this discovery, many studies have investigated the microbiota of different parts of the udder in various conditions. However, few studies have followed the changes that occur in the udder microbiota over time. In this study, the temporal dynamics of the udder microbiota of 10 cows, five with a low somatic cell count (SCC, SCC < 100,000 cells/mL) and five with a high SCC (SCC > 100,000 cells/mL), were followed over 5 months to gather insights into this knowledge gap. Analysis of the temporal changes in the microbial composition of milk from udders with a low SCC revealed a dynamic and diverse microbiota. When an imbalance due to one dominating genus was recorded, the dominant genus quickly vanished, and the high diversity was restored. The genera dominating in the samples with a high SCC remained the dominant genera throughout the whole sampling period. These cows generally displayed a heightened SCC or an intramammary infection in at least one quarter though-out the sampling period. Our results show that the bovine udder has a diverse microbiota, and that the composition and diversity of this community affects udder health with regards to SCC. Understanding what influences the composition and stability of this community has important implications for the understanding, control, and treatment of mastitis.
Sections du résumé
BACKGROUND
BACKGROUND
In recent years, the number of studies concerning microbiota of the intramammary environment has increased rapidly due to the development of high-throughput sequencing technologies that allow mapping of microbiota without culturing. This has revealed that an environment previously thought to be sterile in fact harbours a microbial community. Since this discovery, many studies have investigated the microbiota of different parts of the udder in various conditions. However, few studies have followed the changes that occur in the udder microbiota over time. In this study, the temporal dynamics of the udder microbiota of 10 cows, five with a low somatic cell count (SCC, SCC < 100,000 cells/mL) and five with a high SCC (SCC > 100,000 cells/mL), were followed over 5 months to gather insights into this knowledge gap.
RESULTS
RESULTS
Analysis of the temporal changes in the microbial composition of milk from udders with a low SCC revealed a dynamic and diverse microbiota. When an imbalance due to one dominating genus was recorded, the dominant genus quickly vanished, and the high diversity was restored. The genera dominating in the samples with a high SCC remained the dominant genera throughout the whole sampling period. These cows generally displayed a heightened SCC or an intramammary infection in at least one quarter though-out the sampling period.
CONCLUSION
CONCLUSIONS
Our results show that the bovine udder has a diverse microbiota, and that the composition and diversity of this community affects udder health with regards to SCC. Understanding what influences the composition and stability of this community has important implications for the understanding, control, and treatment of mastitis.
Identifiants
pubmed: 35395785
doi: 10.1186/s42523-022-00177-w
pii: 10.1186/s42523-022-00177-w
pmc: PMC8994269
doi:
Types de publication
Journal Article
Langues
eng
Pagination
26Informations de copyright
© 2022. The Author(s).
Références
Appl Environ Microbiol. 2016 Dec 30;83(2):
pubmed: 27815277
Vet Res. 2003 Sep-Oct;34(5):671-88
pubmed: 14556700
PLoS One. 2013 Apr 25;8(4):e61959
pubmed: 23634219
Microbiome. 2017 Jul 12;5(1):74
pubmed: 28701174
Vet Res. 2003 Sep-Oct;34(5):579-96
pubmed: 14556696
J Dairy Sci. 2018 Jul;101(7):6346-6356
pubmed: 29680645
Vet Res Commun. 2005 Apr;29(3):215-45
pubmed: 15736856
Can J Vet Res. 1987 Jan;51(1):27-31
pubmed: 3552170
PLoS One. 2014 Jan 20;9(1):e85904
pubmed: 24465777
Front Microbiol. 2016 Apr 08;7:480
pubmed: 27242672
PLoS One. 2012;7(10):e47671
pubmed: 23082192
Gut Microbes. 2020 Nov 9;12(1):1667722
pubmed: 31684806
Sci Rep. 2016 Nov 22;6:37565
pubmed: 27874095
Reprod Domest Anim. 2021 Sep;56(9):1165-1175
pubmed: 34008236
J Appl Microbiol. 2006 Nov;101(5):1152-60
pubmed: 17040239
J Dairy Sci. 2011 Jan;94(1):250-61
pubmed: 21183035
Nat Methods. 2016 Jul;13(7):581-3
pubmed: 27214047
Nat Commun. 2020 Jul 14;11(1):3514
pubmed: 32665548
PLoS One. 2019 Nov 14;14(11):e0225001
pubmed: 31725757
Vet Microbiol. 2012 Jun 15;157(3-4):383-90
pubmed: 22266158
Microbiome. 2018 Aug 9;6(1):140
pubmed: 30092815
J Dairy Sci. 2018 Dec;101(12):10605-10625
pubmed: 30292553
Nucleic Acids Res. 2013 Jan;41(Database issue):D590-6
pubmed: 23193283
PLoS Pathog. 2015 Jul 09;11(7):e1004923
pubmed: 26158874
mBio. 2016 Jan 05;7(1):e01725-15
pubmed: 26733066
Vet Res. 2019 Jun 6;50(1):44
pubmed: 31171032
J Dairy Sci. 2018 Oct;101(10):9493-9504
pubmed: 30122416
Animals (Basel). 2022 Feb 13;12(4):
pubmed: 35203168
J Dairy Sci. 2019 Jan;102(1):629-639
pubmed: 30415854
J Dairy Sci. 2017 Apr;100(4):3031-3042
pubmed: 28161185
J Dairy Sci. 1998 Jun;81(6):1545-54
pubmed: 9684160
J Dairy Sci. 2007 Sep;90(9):4483-97
pubmed: 17699070
Front Vet Sci. 2021 May 07;8:656810
pubmed: 34026893
Vet Microbiol. 2017 Aug;207:259-266
pubmed: 28757033
Vet Res. 2017 Apr 17;48(1):25
pubmed: 28412972
J Appl Microbiol. 2014 May;116(5):1084-93
pubmed: 24443828
Sci Rep. 2020 Dec 10;10(1):21608
pubmed: 33303769