Dynamic Ca
CP: Immunology
D614G spike
FRET
SARS CoV2
calcium signaling
conformational dynamics
fluorescence anisotropy
membrane fusion
virus entry
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
19 04 2022
19 04 2022
Historique:
received:
03
08
2021
revised:
10
12
2021
accepted:
25
03
2022
pubmed:
10
4
2022
medline:
23
4
2022
entrez:
9
4
2022
Statut:
ppublish
Résumé
Mutations in the spike protein generated a highly infectious and transmissible D614G variant, which is present in newly evolved fast-spreading variants. The D614G, Alpha, Beta, and Delta spike variants of SARS-CoV-2 appear to expedite membrane fusion process for entry, but the mechanism of spike-mediated fusion is unknown. Here, we reconstituted an in vitro pseudovirus-liposome fusion reaction and report that SARS-CoV-2 wild-type spike is a dynamic Ca
Identifiants
pubmed: 35397208
pii: S2211-1247(22)00452-1
doi: 10.1016/j.celrep.2022.110694
pmc: PMC8993541
pii:
doi:
Substances chimiques
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110694Subventions
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interest The authors declare no competing interests.
Références
Cell. 2021 Jan 7;184(1):64-75.e11
pubmed: 33275900
Nat Med. 2021 Apr;27(4):622-625
pubmed: 33654292
J Cell Sci. 2002 Feb 1;115(Pt 3):599-607
pubmed: 11861766
PLoS Pathog. 2014 Nov 06;10(11):e1004502
pubmed: 25375324
Nat Struct Mol Biol. 2007 Oct;14(10):904-11
pubmed: 17891149
Cell. 2018 Aug 9;174(4):926-937.e12
pubmed: 29961575
Cell Discov. 2020 Dec 22;6(1):96
pubmed: 33349633
Science. 2009 Jan 23;323(5913):474-7
pubmed: 19164740
Science. 2015 Feb 27;347(6225):995-8
pubmed: 25722412
Cell. 2020 Sep 3;182(5):1284-1294.e9
pubmed: 32730807
Nat Biotechnol. 2002 Nov;20(11):1151-4
pubmed: 12355096
Cell. 2020 Oct 29;183(3):739-751.e8
pubmed: 32991842
Cell Host Microbe. 2020 Dec 9;28(6):867-879.e5
pubmed: 33271067
Viruses. 2021 Mar 01;13(3):
pubmed: 33804556
Nature. 2020 Mar;579(7798):270-273
pubmed: 32015507
Science. 2020 Mar 13;367(6483):1260-1263
pubmed: 32075877
Nat Commun. 2020 Nov 26;11(1):6013
pubmed: 33243994
Nature. 2021 Apr;592(7852):116-121
pubmed: 33106671
PLoS Pathog. 2014 Dec 04;10(12):e1004530
pubmed: 25474548
Science. 2020 Nov 13;370(6518):856-860
pubmed: 33082293
PLoS Biol. 2021 Apr 29;19(4):e3001237
pubmed: 33914735
Science. 2021 Aug 6;373(6555):
pubmed: 34168071
Science. 2020 Nov 13;370(6518):861-865
pubmed: 33082294
Mol Cell. 2020 Sep 3;79(5):710-727
pubmed: 32853546
Angew Chem Int Ed Engl. 2016 Dec 23;55(52):16172-16176
pubmed: 27804198
Cell. 2020 Apr 16;181(2):281-292.e6
pubmed: 32155444
Nat Struct Mol Biol. 2020 Oct;27(10):934-941
pubmed: 32737467
ACS Infect Dis. 2020 Feb 14;6(2):250-260
pubmed: 31746195
Curr Biol. 1998 Dec 3;8(24):1335-8
pubmed: 9843688
Cell. 2020 Aug 20;182(4):812-827.e19
pubmed: 32697968
Nature. 2021 Jun;594(7861):88-93
pubmed: 33827113
Sci Rep. 2017 Jan 24;7:41226
pubmed: 28117364
Science. 2020 Sep 25;369(6511):1586-1592
pubmed: 32694201
Science. 2021 Apr 30;372(6541):525-530
pubmed: 33727252
J Mol Biol. 2017 Dec 8;429(24):3875-3892
pubmed: 29056462
PLoS Biol. 2020 Feb 10;18(2):e3000626
pubmed: 32040508
Cell Rep Med. 2022 Mar 11;3(4):100583
pubmed: 35480627
Science. 2014 Nov 7;346(6210):759-63
pubmed: 25298114
Science. 2012 Jun 22;336(6088):1581-4
pubmed: 22653732
Cell. 2020 Apr 16;181(2):271-280.e8
pubmed: 32142651
Nature. 2021 Nov;599(7883):114-119
pubmed: 34488225
J Biol Chem. 2007 Sep 14;282(37):27327-27333
pubmed: 17609211
Science. 2020 Dec 18;370(6523):1464-1468
pubmed: 33184236
Nature. 2021 May;593(7857):130-135
pubmed: 33684923
J Virol. 2020 Jun 16;94(13):
pubmed: 32295925