Performance evaluation of UF-4000 body fluid mode for automated body fluid cell counting.
Automated cell count
Body fluids
Chamber count
Optical microscopy
Sysmex UF-4000
Journal
Clinica chimica acta; international journal of clinical chemistry
ISSN: 1873-3492
Titre abrégé: Clin Chim Acta
Pays: Netherlands
ID NLM: 1302422
Informations de publication
Date de publication:
01 Jun 2022
01 Jun 2022
Historique:
received:
04
04
2022
accepted:
04
04
2022
pubmed:
11
4
2022
medline:
7
6
2022
entrez:
10
4
2022
Statut:
ppublish
Résumé
Cytological analysis of body fluids (BF) provides important information for diagnosis in various medical conditions. We evaluated the analytical performance of the UF-4000 BF mode for ascitic, cerebrospinal, pleural, synovial and continuous ambulatory peritoneal dialysis fluids compared to light microscopy counting (LM). 223 consecutive BF were analyzed by UF-4000 and results were compared using Pearson's correlation, Bland-Altman analysis, and contingence tests at relevant cut-off values. This study also included the evaluation of precision, linearity, and carryover. For white and red blood cells (WBC, RBC) counts in all BF, correlation was excellent with Pearson's coefficients R UF-4000 BF mode is a good alternative to manual LM for BF cell counting. This automated method gives rapid and accurate results which is important for therapeutic decisions.
Sections du résumé
BACKGROUND
BACKGROUND
Cytological analysis of body fluids (BF) provides important information for diagnosis in various medical conditions. We evaluated the analytical performance of the UF-4000 BF mode for ascitic, cerebrospinal, pleural, synovial and continuous ambulatory peritoneal dialysis fluids compared to light microscopy counting (LM).
MATERIALS AND METHODS
METHODS
223 consecutive BF were analyzed by UF-4000 and results were compared using Pearson's correlation, Bland-Altman analysis, and contingence tests at relevant cut-off values. This study also included the evaluation of precision, linearity, and carryover.
RESULTS
RESULTS
For white and red blood cells (WBC, RBC) counts in all BF, correlation was excellent with Pearson's coefficients R
CONCLUSION
CONCLUSIONS
UF-4000 BF mode is a good alternative to manual LM for BF cell counting. This automated method gives rapid and accurate results which is important for therapeutic decisions.
Identifiants
pubmed: 35398022
pii: S0009-8981(22)00122-X
doi: 10.1016/j.cca.2022.04.002
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
152-156Informations de copyright
Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.