Real-time imaging of enzymatic degradation of pretreated maize internodes reveals different cell types have different profiles.
FTIR microspectroscopy
Fluorescence
Hot water pretreatment
Lignocellulose
Microspectrometry
Journal
Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
25
02
2022
revised:
05
04
2022
accepted:
06
04
2022
pubmed:
12
4
2022
medline:
27
4
2022
entrez:
11
4
2022
Statut:
ppublish
Résumé
This work presents a dynamic view of the enzymatic degradation of maize cell walls, and sheds new light on the recalcitrance of hot water pretreated maize stem internodes. Infra-red microspectrometry, mass spectrometry, fluorescence recovery after photobleaching and fluorescence imaging were combined to investigate enzymatic hydrolysis at the cell scale. Depending on their polymer composition and organisation, cell types exhibits different extent and rate of enzymatic degradation. Enzymes act sequentially from the cell walls rich in accessible cellulose to the most recalcitrant cells. This phenomenon can be linked to the heterogeneous distribution of enzymes in the liquid medium and the adsorption/desorption mechanisms that differ with the type of cell.
Identifiants
pubmed: 35405211
pii: S0960-8524(22)00469-2
doi: 10.1016/j.biortech.2022.127140
pii:
doi:
Substances chimiques
Water
059QF0KO0R
Cellulose
9004-34-6
Lignin
9005-53-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
127140Informations de copyright
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