Expression of Oxytocin/Neurophysin I and Oxytocinase in the Equine Conceptus from Day 8 to Day 21 Post-Ovulation.
equine conceptus
glucose transporters
leucyl and cystinyl aminopeptidase
oxytocin/neurophysin I
prostaglandin
Journal
Animals : an open access journal from MDPI
ISSN: 2076-2615
Titre abrégé: Animals (Basel)
Pays: Switzerland
ID NLM: 101635614
Informations de publication
Date de publication:
22 Mar 2022
22 Mar 2022
Historique:
received:
23
02
2022
revised:
16
03
2022
accepted:
20
03
2022
entrez:
12
4
2022
pubmed:
13
4
2022
medline:
13
4
2022
Statut:
epublish
Résumé
Leucyl and cystinyl aminopeptidase (LNPEP/oxytocinase) is an enzyme that metabolizes oxytocin in serum and tissues. The presence of oxytocin/neurophysin I (OXT), oxytocin and LNPEP and their relationship to other genes is unknown in the equine conceptus. Our objective was to characterize gene expression of LNPEP and OXT on D8, 10, 12, 14, 15, 16 and 21 conceptuses in relationship to other genes. Immunohistochemistry, western blot and liquid chromatography with tandem mass spectrometry (LC-MS/MS) were used for identification of oxytocin and LNPEP in D15, 16 and 18 conceptuses. LNPEP was increased at D15 compared to D10, was immunolocalized in the equine trophectoderm and endoderm, and protein was confirmed by LC-MS/MS. Maximal abundance of OXT was at D21, and lowest on D12 and D14, but no protein was identified. OXTR abundance was highest on D14 and D21. LNPEP was correlated with PTGFR and PTGES on D12 and D14-D15, and high expression of PTGES, PTGS2 was found on D14, D15 and D21; PTGFR was found on D8 and D12-21. LNPEP may have a role in prostaglandin regulation and conceptus fixation by decreasing the availability of oxytocin. Further investigation on the role embryonic LNPEP during pregnancy is warranted.
Identifiants
pubmed: 35405789
pii: ani12070799
doi: 10.3390/ani12070799
pmc: PMC8996865
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : University of Saskatchewan
ID : G00016114
Références
Prostaglandins. 1992 Jan;43(1):55-9
pubmed: 1546173
Peptides. 1980 Winter;1(4):315-24
pubmed: 6892474
Asian-Australas J Anim Sci. 2017 May;30(5):643-652
pubmed: 27764917
Theriogenology. 1984 Oct;22(4):401-8
pubmed: 16725972
Placenta. 2018 Aug;68:23-32
pubmed: 30055666
Sci Rep. 2020 Feb 7;10(1):2089
pubmed: 32034259
J Reprod Fertil. 1989 Sep;87(1):331-6
pubmed: 2621704
Biol Reprod. 2004 Feb;70(2):391-9
pubmed: 14561653
J Reprod Fertil. 1998 Mar;112(2):337-46
pubmed: 9640273
Electrophoresis. 2007 Jun;28(12):2080-94
pubmed: 17486657
J Reprod Fertil Suppl. 2000;(56):281-7
pubmed: 20681139
Cell Tissue Res. 1979 Mar 19;197(2):325-36
pubmed: 436149
Theriogenology. 2019 Jan 1;123:132-138
pubmed: 30300810
J Am Vet Med Assoc. 2007 Dec 15;231(12):1864-7
pubmed: 18081527
Electrophoresis. 2018 Sep;39(18):2332-2339
pubmed: 29644703
Reprod Biol Endocrinol. 2004 Nov 24;2:77
pubmed: 15560851
J Anim Sci. 2015 Jul;93(7):3364-76
pubmed: 26440005
Biochim Biophys Acta. 2005 Aug 1;1751(1):9-18
pubmed: 16054015
Biol Reprod. 2012 Jul 01;87(1):9, 1-7
pubmed: 22517622
Nature. 1982 May 20;297(5863):225-7
pubmed: 7078636
J Reprod Fertil. 1997 Jan;109(1):137-44
pubmed: 9068425
Prostaglandins. 1992 Jan;43(1):61-5
pubmed: 1546174
Anal Biochem. 2017 May 15;525:46-53
pubmed: 28237256
Theriogenology. 1998 Nov;50(7):989-99
pubmed: 10734418
J Reprod Fertil Suppl. 2000;(56):289-96
pubmed: 20681140
Anim Reprod Sci. 2011 Nov;129(1-2):67-72
pubmed: 22024365
Theriogenology. 1999 Apr 1;51(5):1017-25
pubmed: 10729023
J Reprod Fertil. 1999 Jul;116(2):315-20
pubmed: 10615256
Theriogenology. 1994;42(7):1069-83
pubmed: 16727611
Animals (Basel). 2021 Apr 20;11(4):
pubmed: 33924239
Biol Reprod. 2009 Jan;80(1):94-104
pubmed: 18753604
Reproduction. 2001 May;121(5):771-5
pubmed: 11427165
Equine Vet J. 1999 Mar;31(2):174-5
pubmed: 10213431
J Anim Sci Biotechnol. 2015 Dec 02;6:50
pubmed: 26635959
Endocrinology. 1992 Mar;130(3):1185-92
pubmed: 1537285
Regul Pept. 2004 Oct 15;122(2):85-9
pubmed: 15380925
J Reprod Fertil. 1992 Jul;95(2):535-8
pubmed: 1518007
Domest Anim Endocrinol. 1999 Apr;16(3):183-92
pubmed: 10343920
Sci Rep. 2018 Mar 27;8(1):5249
pubmed: 29588480
J Endocrinol. 1991 Sep;130(3):443-9
pubmed: 1940718
Theriogenology. 1984 Jul;22(1):83-95
pubmed: 16725939
J Biol Chem. 1994 Feb 25;269(8):6089-92
pubmed: 8119954
Reprod Biol Endocrinol. 2003 Nov 10;1:92
pubmed: 14613532
Theriogenology. 2015 Mar 1;83(4):730-8
pubmed: 25523835
Biochim Biophys Acta. 2005 Aug 1;1751(1):19-25
pubmed: 15894523
Reprod Fertil Dev. 2012;24(5):641-8
pubmed: 22697114
Biol Reprod. 2010 Jul;83(1):102-13
pubmed: 20335638
Endocrinology. 1983 Dec;113(6):2302-4
pubmed: 6641635
Biol Reprod. 2006 Feb;74(2):337-43
pubmed: 16237147
Equine Vet J. 2011 Jan;43(1):99-105
pubmed: 21143640
Reprod Biol Endocrinol. 2017 Jan 5;15(1):4
pubmed: 28056994
Horm Metab Res. 2005 Jan;37(1):4-9
pubmed: 15702431
Proteomics. 2017 Sep;17(17-18):
pubmed: 28782881
Reproduction. 2009 Dec;138(6):953-60
pubmed: 19710203
Folia Histochem Cytobiol. 1985;23(1-2):81-4
pubmed: 3862620
Biol Reprod. 2004 Oct;71(4):1120-7
pubmed: 15163615
Biochim Biophys Acta Mol Basis Dis. 2020 Feb 1;1866(2):165359
pubmed: 30593896
Reproduction. 2002 Feb;123(2):261-8
pubmed: 11866693
J Reprod Fertil. 1999 Sep;117(1):135-42
pubmed: 10645254
Reprod Fertil Dev. 2015 Jun;27(5):834-45
pubmed: 24679480
Theriogenology. 2008 Feb;69(3):366-75
pubmed: 18037481
Neuropeptides. 2000 Jun-Aug;34(3-4):173-80
pubmed: 11021977
J Neurosci. 1988 Nov;8(11):3967-77
pubmed: 3183709