5-Azacytidine Downregulates the Proliferation and Migration of Hepatocellular Carcinoma Cells In Vitro and In Vivo by Targeting miR-139-5p/ROCK2 Pathway.

5-azacytidine E2F1 MMP-2 ROCK2 cell cycle hepatocellular carcinoma miR-139-5p migration

Journal

Cancers
ISSN: 2072-6694
Titre abrégé: Cancers (Basel)
Pays: Switzerland
ID NLM: 101526829

Informations de publication

Date de publication:
23 Mar 2022
Historique:
received: 30 12 2021
revised: 14 02 2022
accepted: 18 03 2022
entrez: 12 4 2022
pubmed: 13 4 2022
medline: 13 4 2022
Statut: epublish

Résumé

For hepatocellular carcinoma (HCC), effective therapeutic approaches are lacking. As aberrant gene methylation is a major contributor to HCC development, demethylating drugs such as 5-azacytidine (5-Aza) have been proposed. As most 5-Aza mechanisms of action are unknown, we investigated its phenotypic/molecular effects. 5-Aza effects were examined in the human HCC cell lines JHH-6/HuH-7 and in the rat cell-line N1-S1. We also employed a xenograft mouse model (HuH-7), a zebrafish model (JHH-6), and an orthotopic syngeneic rat model (N1-S1) of HCC. 5-Aza downregulated cell viability/growth/migration/adhesion by upregulating miR-139-5p, which in turn downregulated ROCK2/cyclin D1/E2F1 and increased p27 We demonstrate for the first time that 5-Aza can impair HCC development via upregulation of miR-139-5p, which in turn impairs the ROCK2/cyclin D1/E2F1/cyclin B1 pro-proliferative pathway and the ROCK2/MMP-2 pro-migratory pathway. Thus, we provide novel information about 5-Aza mechanisms of action and deepen the knowledge about the crosstalk among ROCK2/cyclin D1/E2F1/cyclin B1/p27

Sections du résumé

BACKGROUND BACKGROUND
For hepatocellular carcinoma (HCC), effective therapeutic approaches are lacking. As aberrant gene methylation is a major contributor to HCC development, demethylating drugs such as 5-azacytidine (5-Aza) have been proposed. As most 5-Aza mechanisms of action are unknown, we investigated its phenotypic/molecular effects.
METHODS METHODS
5-Aza effects were examined in the human HCC cell lines JHH-6/HuH-7 and in the rat cell-line N1-S1. We also employed a xenograft mouse model (HuH-7), a zebrafish model (JHH-6), and an orthotopic syngeneic rat model (N1-S1) of HCC.
RESULTS RESULTS
5-Aza downregulated cell viability/growth/migration/adhesion by upregulating miR-139-5p, which in turn downregulated ROCK2/cyclin D1/E2F1 and increased p27
CONCLUSION CONCLUSIONS
We demonstrate for the first time that 5-Aza can impair HCC development via upregulation of miR-139-5p, which in turn impairs the ROCK2/cyclin D1/E2F1/cyclin B1 pro-proliferative pathway and the ROCK2/MMP-2 pro-migratory pathway. Thus, we provide novel information about 5-Aza mechanisms of action and deepen the knowledge about the crosstalk among ROCK2/cyclin D1/E2F1/cyclin B1/p27

Identifiants

pubmed: 35406401
pii: cancers14071630
doi: 10.3390/cancers14071630
pmc: PMC8996928
pii:
doi:

Types de publication

Journal Article

Langues

eng

Subventions

Organisme : Fondazione Benefica Kathleen Foreman Casali of Trieste, Italy
ID : no number
Organisme : Beneficentia Stiftung" of Vaduz Liechtenstein
ID : no number
Organisme : Slovenian Research Agency (ARRS)
ID : P3-0003
Organisme : Progetto HEaD "HIGHER EDUCATION AND DEVELOPMENT" UNITS, Fondo sociale Europeo 2014/2020, Asse 3, Programma specifico 25/15
ID : FP168562300
Organisme : Italian Ministry of Foreign Affairs and International Cooperation
ID : VN21GR01

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Auteurs

Federica Tonon (F)

Department of Life Sciences, Cattinara University Hospital, Trieste University, Strada di Fiume 447, I-34149 Trieste, Italy.

Maja Cemazar (M)

Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, SI-1000 Ljubljana, Slovenia.
Faculty of Health Sciences, University of Primorska, Polje 42, SI-6310 Izola, Slovenia.

Urska Kamensek (U)

Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, SI-1000 Ljubljana, Slovenia.

Cristina Zennaro (C)

Department of Medical, Surgical and Health Sciences, University of Trieste, Cattinara Hospital, Strada di Fiume 447, I-34149 Trieste, Italy.

Gabriele Pozzato (G)

Department of Medical, Surgical and Health Sciences, University of Trieste, Cattinara Hospital, Strada di Fiume 447, I-34149 Trieste, Italy.

Sergio Caserta (S)

Department of Chemical, Materials and Industrial Production Engineering, University of Naples "Federico II", Piazzale V. Tecchio 80, I-80125 Naples, Italy.
CEINGE Advanced Biotechnologies, via Gaetano Salvatore, 486, I-80145 Napoli, Italy.

Flora Ascione (F)

Department of Chemical, Materials and Industrial Production Engineering, University of Naples "Federico II", Piazzale V. Tecchio 80, I-80125 Naples, Italy.

Mario Grassi (M)

Department of Engineering and Architecture, University of Trieste, Via Valerio 6/A, I-34127 Trieste, Italy.

Stefano Guido (S)

Department of Chemical, Materials and Industrial Production Engineering, University of Naples "Federico II", Piazzale V. Tecchio 80, I-80125 Naples, Italy.
CEINGE Advanced Biotechnologies, via Gaetano Salvatore, 486, I-80145 Napoli, Italy.

Cinzia Ferrari (C)

Department of Clinic-Surgical Sciences, Laboratory of Experimental Surgery and Animal Facility, University of Pavia, Via Ferrata 9, I-27100 Pavia, Italy.

Laura Cansolino (L)

Department of Clinic-Surgical Sciences, Laboratory of Experimental Surgery and Animal Facility, University of Pavia, Via Ferrata 9, I-27100 Pavia, Italy.

Francesco Trotta (F)

Department of General Surgery, Maggiore Hospital, Largo Donatori del Sangue 1, I-26900 Lodi, Italy.

Biljana Grcar Kuzmanov (BG)

Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, SI-1000 Ljubljana, Slovenia.

Giancarlo Forte (G)

International Clinical Research Center (ICRC) of St Anne's University Hospital, CZ-65691 Brno, Czech Republic.

Fabiana Martino (F)

International Clinical Research Center (ICRC) of St Anne's University Hospital, CZ-65691 Brno, Czech Republic.

Francesca Perrone (F)

Department of Life Sciences, Cattinara University Hospital, Trieste University, Strada di Fiume 447, I-34149 Trieste, Italy.
Department of Paediatrics, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge CB2 0QQ, UK.

Riccardo Bomben (R)

Clinical and Experimental Onco-Haematology Unit, Centro di Riferimento Oncologico, Istituto di Ricovero a Cura a Carattere Scientifico IRCCS, 33081 Aviano, Italy.

Valter Gattei (V)

Clinical and Experimental Onco-Haematology Unit, Centro di Riferimento Oncologico, Istituto di Ricovero a Cura a Carattere Scientifico IRCCS, 33081 Aviano, Italy.

Nicola Elvassore (N)

Industrial Engineering Department, University of Padova, Via Francesco Marzolo, 9, I-35131 Padova, Italy.

Erminio Murano (E)

Nealys SRL, Via Flavia 23/1, I-34148 Trieste, Italy.

Nhung Hai Truong (NH)

Stem Cell Research and Application Laboratory, VNUHCM, University of Science, Ho Chi Minh City 72711, Vietnam.

Michael Olson (M)

Department of Chemistry and Biology, X University, MaRS Discovery District, West Tower 661 University Avenue, Toronto, ON M5G 1M1, Canada.

Rossella Farra (R)

Department of Life Sciences, Cattinara University Hospital, Trieste University, Strada di Fiume 447, I-34149 Trieste, Italy.

Gabriele Grassi (G)

Department of Life Sciences, Cattinara University Hospital, Trieste University, Strada di Fiume 447, I-34149 Trieste, Italy.

Barbara Dapas (B)

Department of Life Sciences, Cattinara University Hospital, Trieste University, Strada di Fiume 447, I-34149 Trieste, Italy.

Classifications MeSH