Tolerance of Human Fibroblasts to Benfo-Oxythiamine In Vitro.
SARS-CoV-2
benfo-oxythiamine
fibroblast
virus replication
Journal
International journal of environmental research and public health
ISSN: 1660-4601
Titre abrégé: Int J Environ Res Public Health
Pays: Switzerland
ID NLM: 101238455
Informations de publication
Date de publication:
30 03 2022
30 03 2022
Historique:
received:
18
02
2022
revised:
17
03
2022
accepted:
25
03
2022
entrez:
12
4
2022
pubmed:
13
4
2022
medline:
14
4
2022
Statut:
epublish
Résumé
To explore the potential application of B-OT in the aspiration tract. We conceived and optimized an in vitro model simulating the mouth-washing process to assess tolerance to B-OT on primary human gingival fibroblasts. Cells derived from 4 unrelated donors were flushed with medium containing drugs of various concentration for one minute twice daily for 3 days. No effect was seen on the cells up to 1000 µM B-OT. In addition, we treated the cells with B-OT permanently in medium, corresponding to a systemic treatment. No effect was seen by 10 µM B-OT and only a slight reduction (approximately 10%) was seen by 100 µM B-OT. Our results suggest good tolerance of oral cells for B-OT, favoring the further development of this antiviral reagent as a mouth-washing solution and nasal spray.
Identifiants
pubmed: 35409800
pii: ijerph19074112
doi: 10.3390/ijerph19074112
pmc: PMC8998213
pii:
doi:
Substances chimiques
Antiviral Agents
0
Oxythiamine
136-16-3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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