Correlations of Salivary and Blood Glucose Levels among Six Saliva Collection Methods.
blood glucose
correlation
diabetes mellitus
saliva collection method
salivary glucose
Journal
International journal of environmental research and public health
ISSN: 1660-4601
Titre abrégé: Int J Environ Res Public Health
Pays: Switzerland
ID NLM: 101238455
Informations de publication
Date de publication:
30 03 2022
30 03 2022
Historique:
received:
08
03
2022
revised:
27
03
2022
accepted:
29
03
2022
entrez:
12
4
2022
pubmed:
13
4
2022
medline:
14
4
2022
Statut:
epublish
Résumé
Saliva has been studied as a better indicator of disorders and diseases than blood. Specifically, the salivary glucose level is considered to be an indicator of diabetes mellitus (DM). However, saliva collection methods can affect the salivary glucose level, thereby affecting the correlation between salivary glucose and blood glucose. Therefore, this study aims to identify an ideal saliva collection method and to use this method to determine the population and individual correlations between salivary glucose and blood glucose levels in DM patients and healthy controls. Finally, an analysis of the stability of the individual correlations is conducted. This study included 40 age-matched DM patients and 40 healthy controls. In the fasting state, saliva was collected using six saliva collection methods, venous blood was collected simultaneously from each study participant, and both samples were analyzed at the same time using glucose oxidase peroxidase. A total of 20 DM patients and 20 healthy controls were arbitrarily selected from the above participants for one week of daily testing. The correlations between salivary glucose and blood glucose before and after breakfast were analyzed. Finally, 10 DM patients and 10 healthy controls were arbitrarily selected for one month of daily testing to analyze the stability of individual correlations. Salivary glucose levels were higher in DM patients than healthy controls for the six saliva collection methods. Compared with unstimulated saliva, stimulated saliva had decreased glucose level and increased salivary flow. In addition, unstimulated parotid salivary glucose was most correlated with blood glucose level (R Unstimulated parotid salivary glucose level is the highest and is most correlated with blood glucose level, which can be accurately used to distinguish DM patients. Meanwhile, the correlation between salivary glucose and blood glucose was found to be relatively high and stable before breakfast. In general, the unstimulated parotid salivary glucose before breakfast presents an ideal saliva collecting method with which to replace blood-glucose use to detect DM, which provides a reference for the prediction of DM.
Sections du résumé
BACKGROUND
Saliva has been studied as a better indicator of disorders and diseases than blood. Specifically, the salivary glucose level is considered to be an indicator of diabetes mellitus (DM). However, saliva collection methods can affect the salivary glucose level, thereby affecting the correlation between salivary glucose and blood glucose. Therefore, this study aims to identify an ideal saliva collection method and to use this method to determine the population and individual correlations between salivary glucose and blood glucose levels in DM patients and healthy controls. Finally, an analysis of the stability of the individual correlations is conducted.
METHODS
This study included 40 age-matched DM patients and 40 healthy controls. In the fasting state, saliva was collected using six saliva collection methods, venous blood was collected simultaneously from each study participant, and both samples were analyzed at the same time using glucose oxidase peroxidase. A total of 20 DM patients and 20 healthy controls were arbitrarily selected from the above participants for one week of daily testing. The correlations between salivary glucose and blood glucose before and after breakfast were analyzed. Finally, 10 DM patients and 10 healthy controls were arbitrarily selected for one month of daily testing to analyze the stability of individual correlations.
RESULTS
Salivary glucose levels were higher in DM patients than healthy controls for the six saliva collection methods. Compared with unstimulated saliva, stimulated saliva had decreased glucose level and increased salivary flow. In addition, unstimulated parotid salivary glucose was most correlated with blood glucose level (R
CONCLUSION
Unstimulated parotid salivary glucose level is the highest and is most correlated with blood glucose level, which can be accurately used to distinguish DM patients. Meanwhile, the correlation between salivary glucose and blood glucose was found to be relatively high and stable before breakfast. In general, the unstimulated parotid salivary glucose before breakfast presents an ideal saliva collecting method with which to replace blood-glucose use to detect DM, which provides a reference for the prediction of DM.
Identifiants
pubmed: 35409805
pii: ijerph19074122
doi: 10.3390/ijerph19074122
pmc: PMC8999001
pii:
doi:
Substances chimiques
Blood Glucose
0
Oxidoreductases
EC 1.-
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Références
PLoS One. 2020 Mar 17;15(3):e0223461
pubmed: 32182246
Biosensors (Basel). 2021 Jun 09;11(6):
pubmed: 34207533
Cell Rep. 2020 Nov 17;33(7):108402
pubmed: 33207190
Pak J Pharm Sci. 2001 Jan;14(1):33-7
pubmed: 16414850
Heliyon. 2019 Mar 02;5(3):e01286
pubmed: 30886931
J Oral Maxillofac Res. 2012 Oct 01;3(3):e3
pubmed: 24422015
J Oral Maxillofac Pathol. 2017 Sep-Dec;21(3):334-339
pubmed: 29391704
Food Res Int. 2021 Jun;144:110319
pubmed: 34053524
Biomed Pharmacother. 2021 Oct;142:111977
pubmed: 34364042
J Dent. 2019 Jan;80 Suppl 1:S3-S12
pubmed: 30696553
Cureus. 2019 Sep 12;11(9):e5634
pubmed: 31700737
Braz Oral Res. 2020 Dec 18;35:e016
pubmed: 33331408
Medicine (Baltimore). 2005 Nov;84(6):363-376
pubmed: 16267411
J Gerontol A Biol Sci Med Sci. 2021 Sep 13;76(10):1839-1845
pubmed: 33522564
Sci Rep. 2021 Mar 19;11(1):6406
pubmed: 33742017
Cardiovasc Res. 1997 Apr;34(1):55-68
pubmed: 9217873
Diabetes Metab Syndr Obes. 2021 Jul 01;14:3001-3015
pubmed: 34234496
J Oral Sci. 2020 Dec 23;63(1):22-26
pubmed: 33116006
Int J Dent. 2020 Mar 12;2020:4178674
pubmed: 32256594
Patient Prefer Adherence. 2021 Feb 10;15:283-298
pubmed: 33603347
Sci Rep. 2021 Apr 7;11(1):7663
pubmed: 33828136
Chem Soc Rev. 2013 Apr 21;42(8):3184-96
pubmed: 23420144
Contemp Clin Dent. 2014 Jul;5(3):312-7
pubmed: 25191065
Br J Psychiatry. 2021 Jul;219(1):375-382
pubmed: 33407970
Future Med Chem. 2020 Apr;12(8):645-647
pubmed: 32180439
Clin Oral Investig. 2021 Nov;25(11):6139-6146
pubmed: 33786648
Expert Opin Investig Drugs. 2021 Feb;30(2):85-93
pubmed: 33327794
BMC Infect Dis. 2022 Jan 19;22(1):64
pubmed: 35045811
J Diabetes Sci Technol. 2022 Jan;16(1):168-214
pubmed: 34120487
Adv Drug Deliv Rev. 2018 Jan 15;124:16-33
pubmed: 29108861
Lab Chip. 2018 Aug 21;18(17):2632-2641
pubmed: 30063233
Int J Paediatr Dent. 1998 Mar;8(1):29-33
pubmed: 9558543
NMR Biomed. 2009 Jul;22(6):577-84
pubmed: 19259987
Physiol Rev. 2002 Jul;82(3):569-600
pubmed: 12087129
Sci Rep. 2018 Feb 19;8(1):3220
pubmed: 29459715
J Oral Biosci. 2019 Dec;61(4):201-206
pubmed: 31655176
J Int Soc Prev Community Dent. 2017 Jan-Feb;7(1):28-33
pubmed: 28316946
J Diabetes Complications. 2021 Aug;35(8):107929
pubmed: 33902999
J Contemp Dent Pract. 2019 Apr 1;20(4):494-498
pubmed: 31308283
Oral Dis. 2013 Apr;19(3):219-29
pubmed: 22994880
Eur J Prev Cardiol. 2022 Jan 11;28(16):1858-1860
pubmed: 34583387
N Engl J Med. 1989 Apr 6;320(14):881-6
pubmed: 2648146
Autoimmun Rev. 2018 Jan;17(1):53-72
pubmed: 29108826
Diabetes Metab Syndr Obes. 2012;5:149-54
pubmed: 22923999
Biomed Res Int. 2018 May 9;2018:6572381
pubmed: 29854777