Trade-offs between male fertility reduction and selected growth factors or the klotho response in a lipopolysaccharide-dependent mouse model.
Antidepressant-like substances
Growth factors
Klotho
LPS
Testis
Journal
Toxicological research
ISSN: 1976-8257
Titre abrégé: Toxicol Res
Pays: Singapore
ID NLM: 101483324
Informations de publication
Date de publication:
Apr 2022
Apr 2022
Historique:
received:
21
01
2021
revised:
01
04
2021
accepted:
20
04
2021
entrez:
13
4
2022
pubmed:
14
4
2022
medline:
14
4
2022
Statut:
epublish
Résumé
The increasing number of depression cases leads to a greater need for new antidepressant treatment development. It is postulated that antidepressants may harm male fertility, but the cellular mechanism is still poorly understood. The role of growth factors and klotho protein in maintaining normal male reproductive function is well documented. Hence, the study aimed to investigate the effect of the antidepressant drug - imipramine (tricyclic AD), and other substances with antidepressant potential (ALS), administered in combination or in combination with LPS (an animal model of depression) on gene expression and protein synthesis of IGF-2 (insulin-like growth factor 2), TGF-β1 (transforming growth factor β1), NGF (nerve growth factor), KGF (keratinocyte growth factor) and protein synthesis of VEGF-A (vascular endothelial growth factor A), IGF-IR (insulin-like growth factor receptor 1), EGFR (epidermal growth factor receptor) and klotho in the testis of mice. Mice were injected intraperitoneally with selected ALS and LPS or 10% DMSO (controls) (n = 7/group) once a day for 14 days. Animals were decapitated and testes collected for RNA and protein purification. PCR and western blot methods were employed for the evaluation of growth factors and klotho expression. The results obtained indicated a decreased level of most of the analyzed genes and proteins, except KGF; its expression increased after treatment with MTEP and IMI administrated individually and after NS-398, and IMI in combination with LPS. Our results may suggest that the tested ALS and LPS can contribute to a reduction of male fertility, but NS-398, IMI, and IMI+NS-398 may also act as stimulants after LPS.
Identifiants
pubmed: 35415080
doi: 10.1007/s43188-021-00098-x
pii: 98
pmc: PMC8960506
doi:
Types de publication
Journal Article
Langues
eng
Pagination
175-186Informations de copyright
© The Author(s) 2021.
Déclaration de conflit d'intérêts
Conflict of interestAll authors declare that there is no conflict of interests.
Références
J Mol Histol. 2007 Jun;38(3):207-14
pubmed: 17492480
Biochem Biophys Res Commun. 2001 Feb 2;280(4):1015-20
pubmed: 11162628
J Biol Chem. 2000 Jun 30;275(26):20204-9
pubmed: 10777507
Biol Reprod. 2008 Oct;79(4):738-47
pubmed: 18614701
J Vet Med Sci. 2002 Nov;64(11):967-71
pubmed: 12499679
Endocrinology. 2003 Aug;144(8):3368-75
pubmed: 12865315
Gen Comp Endocrinol. 2002 Apr;126(2):165-74
pubmed: 12030772
Endocrine. 2004 Dec;25(3):229-34
pubmed: 15758250
Fertil Steril. 1999 Aug;72(2):269-75
pubmed: 10438994
Endocrinology. 1992 Dec;131(6):3091-9
pubmed: 1446643
Brain Res. 2020 May 1;1734:146741
pubmed: 32088181
Neuroimmunomodulation. 2014;21(1):52-63
pubmed: 24281669
J Biol Chem. 2000 Jun 16;275(24):18297-301
pubmed: 10748057
Fertil Steril. 2018 May;109(5):879-887
pubmed: 29778387
J Endocrinol Invest. 1995 Mar;18(3):186-93
pubmed: 7615904
J Cell Biol. 1998 Dec 14;143(6):1705-12
pubmed: 9852161
Science. 1983 Feb 25;219(4587):983-5
pubmed: 6823562
Curr Neuropharmacol. 2009 Dec;7(4):331-6
pubmed: 20514212
Tumour Biol. 2013 Jun;34(3):1517-22
pubmed: 23456767
Am J Obstet Gynecol. 2016 Sep;215(3):314.e1-5
pubmed: 26827878
Int J Androl. 2003 Jun;26(3):147-60
pubmed: 12755993
Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11709-14
pubmed: 9751730
Pharmacol Rep. 2013;65(6):1506-11
pubmed: 24552998
Psychoneuroendocrinology. 2000 Jan;25(1):37-51
pubmed: 10633534
J Nephrol. 2014 Oct;27(5):577-85
pubmed: 24760622
Theriogenology. 2019 Mar 1;126:199-205
pubmed: 30579142
Reprod Domest Anim. 2014 Dec;49(6):970-6
pubmed: 25263304
Organogenesis. 2008 Oct;4(4):247-56
pubmed: 19337405
Reprod Domest Anim. 2015 Dec;50(6):918-25
pubmed: 26392300
Basic Clin Androl. 2014 Aug 18;24:12
pubmed: 25780585
Behav Brain Res. 2019 Sep 16;370:111961
pubmed: 31121219
Reprod Fertil Dev. 2016 Mar;28(3):369-74
pubmed: 25066043
Arch Androl. 1998 Mar-Apr;40(2):133-46
pubmed: 9507746
PLoS One. 2011;6(6):e20719
pubmed: 21673960
J Androl. 2012 Jan-Feb;33(1):66-73
pubmed: 21273504
Chonnam Med J. 2018 May;54(2):101-112
pubmed: 29854675
J Urol. 1995 Aug;154(2 Pt 1):576-9
pubmed: 7609140
Dev Biol. 2001 Jan 1;229(1):141-62
pubmed: 11133160
Int Rev Cytol. 1998;183:1-94
pubmed: 9666565
Curr Pharm Des. 2008;14(14):1452-65
pubmed: 18537668
Anim Reprod Sci. 2009 Nov;116(1-2):155-61
pubmed: 19427141
Reprod Biol Endocrinol. 2010 Dec 02;8:148
pubmed: 21126344
Biol Reprod. 2000 Dec;63(6):1617-28
pubmed: 11090428
Blood. 2000 Mar 15;95(6):2052-8
pubmed: 10706874
Biol Reprod. 2000 Jul;63(1):229-41
pubmed: 10859264
Biochim Biophys Acta. 2002 May 3;1575(1-3):63-74
pubmed: 12020820
Endocrine. 1996 Dec;5(3):247-55
pubmed: 21153075
Mol Mar Biol Biotechnol. 1997 Jun;6(2):144-51
pubmed: 9200841
Urology. 2007 Jan;69(1):185.e5-7
pubmed: 17270655
Cell Struct Funct. 2004 Dec;29(4):91-9
pubmed: 15665504
Folia Histochem Cytobiol. 2011;49(1):55-61
pubmed: 21526490
Pharmacol Rep. 2010 Nov-Dec;62(6):1186-90
pubmed: 21273676
J Endocrinol. 1997 Aug;154(2):201-9
pubmed: 9291830
Immunology. 2017 May;151(1):1-15
pubmed: 28112808
Fertil Steril. 2002 Dec;78(6):1164-9
pubmed: 12477505
J Clin Endocrinol Metab. 2015 Oct;100(10):E1308-18
pubmed: 26280509
Nature. 1997 Nov 6;390(6655):45-51
pubmed: 9363890
Biol Reprod. 2007 Jun;76(6):1071-80
pubmed: 17314317
Biol Reprod. 2003 Sep;69(3):985-94
pubmed: 12773425
Ageing Res Rev. 2009 Jan;8(1):43-51
pubmed: 19022406