Ultrasensitive PCR-Free detection of whole virus genome by electrochemiluminescence.
DNA sensor
Electrochemiluminescence
Nucleic acid detection
Point-of-Care sensors
Virus
Journal
Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289
Informations de publication
Date de publication:
01 Aug 2022
01 Aug 2022
Historique:
received:
06
12
2021
revised:
23
02
2022
accepted:
04
03
2022
pubmed:
14
4
2022
medline:
18
5
2022
entrez:
13
4
2022
Statut:
ppublish
Résumé
Detection of nucleic acids is crucial in many medical applications, and in particular for monitoring infectious diseases, as it has become perfectly clear after the pandemic infection of COVID-19. In this context, the development of innovative detection methods based on signal-amplification rather than analyte-amplification represents a significant breakthrough compared to existing PCR-based methodologies, allowing the development of new nucleic acid detection technologies suitable to be integrated in portable and low-cost sensor devices while keeping high sensitivities, thus enabling massive diagnostic screening. In this work, we present a novel molecular sensor for the ultrasensitive PCR-free detection of Hepatitis B Virus (HBV) based on electrochemiluminescence (ECL). Thanks to the combination of surface cooperative hybridization scheme with ECL detection strategy, our novel DNA sensor is able to detect HBV genome - both synthetic and extracted - with the unprecedented limit of detection (LoD) of 0.05 cps μL
Identifiants
pubmed: 35417851
pii: S0956-5663(22)00205-6
doi: 10.1016/j.bios.2022.114165
pmc: PMC9759511
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
114165Informations de copyright
Copyright © 2022 Elsevier B.V. All rights reserved.
Références
Colloids Surf B Biointerfaces. 2020 Mar;187:110648
pubmed: 31767411
Anal Chem. 2018 Nov 6;90(21):12959-12963
pubmed: 30399650
J Clin Microbiol. 1992 Aug;30(8):1913-6
pubmed: 1500493
Lancet. 1981 Oct 10;2(8250):765-8
pubmed: 6116901
ACS Sens. 2018 Sep 28;3(9):1690-1697
pubmed: 30132653
Anal Bioanal Chem. 2016 Oct;408(25):7129-36
pubmed: 27113462
Anal Chem. 2009 Dec 1;81(23):9807-11
pubmed: 19891459
Analyst. 2017 Jun 21;142(12):2090-2093
pubmed: 28425520
Inorg Chem. 1997 Mar 12;36(6):962-965
pubmed: 11669656
Angew Chem Int Ed Engl. 2020 Dec 1;59(49):21858-21863
pubmed: 33000888
Adv Sci (Weinh). 2020 May 27;7(13):1903661
pubmed: 32670752
Nat Biomed Eng. 2020 Dec;4(12):1159-1167
pubmed: 33273713
Clin Microbiol Rev. 2006 Jan;19(1):165-256
pubmed: 16418529
Anal Chem. 2015 Dec 15;87(24):12372-9
pubmed: 26564425
Lab Chip. 2017 Aug 8;17(16):2713-2739
pubmed: 28702608
Lancet. 2015 Oct 17;386(10003):1546-55
pubmed: 26231459
Nat Commun. 2020 Dec 2;11(1):6176
pubmed: 33268779
Anal Chim Acta. 2016 Apr 15;916:92-101
pubmed: 27016443
Anal Chem. 2011 Mar 1;83(5):1815-21
pubmed: 21280583
ACS Sens. 2017 Jul 28;2(7):876-891
pubmed: 28750519
Anal Chem. 2011 Feb 15;83(4):1300-6
pubmed: 21247195
Anal Bioanal Chem. 2019 Jul;411(19):4375-4382
pubmed: 31020369
Nanoscale. 2010 Apr;2(4):606-12
pubmed: 20644766
Dalton Trans. 2016 Sep 14;45(34):13261-76
pubmed: 27426487
Biosens Bioelectron. 2017 May 15;91:456-464
pubmed: 28064131
Anal Chem. 2021 Aug 3;93(30):10397-10402
pubmed: 34213888
RSC Adv. 2018 Jan 8;8(4):1820-1825
pubmed: 35542626
Anal Bioanal Chem. 2016 Oct;408(25):7105-11
pubmed: 27108278
Chem Sci. 2020 Sep 14;11(38):10496-10500
pubmed: 34123186
Anal Chem. 2015 Oct 6;87(19):9777-85
pubmed: 26393525
Anal Chem. 2020 Jan 7;92(1):431-454
pubmed: 31679341
ACS Biomater Sci Eng. 2019 May 13;5(5):2587-2594
pubmed: 33405764
Anal Chem. 2015 Sep 1;87(17):9009-15
pubmed: 26258588
Anal Chem. 2020 Jan 7;92(1):524-534
pubmed: 31789502
Biosens Bioelectron. 2021 Apr 15;178:113015
pubmed: 33493896
Sci Rep. 2012;2:867
pubmed: 23162691
Biosens Bioelectron. 2020 Apr 1;153:112049
pubmed: 32056663
Anal Chem. 2018 Mar 6;90(5):3013-3018
pubmed: 29433314
J Clin Microbiol. 2007 Mar;45(3):828-34
pubmed: 17229858
Chem Commun (Camb). 2020 Sep 7;56(69):10022-10025
pubmed: 32728680
Nat Commun. 2020 May 29;11(1):2668
pubmed: 32472057
J Am Chem Soc. 2005 Oct 19;127(41):14144-5
pubmed: 16218583
Anal Chem. 2019 May 7;91(9):5824-5833
pubmed: 30917656
Nano Today. 2021 Apr;37:101092
pubmed: 33584847