Impact of 16S rRNA Single Nucleotide Polymorphisms on Mycoplasma genitalium Organism Load with Doxycycline Treatment.

Anti-Bacterial Agents / pharmacology Doxycycline / pharmacology Drug Resistance, Bacterial Humans Male Mycoplasma Infections / drug therapy Mycoplasma genitalium / drug effects Polymorphism, Single Nucleotide Prevalence RNA, Ribosomal, 16S / genetics
16S rRNA gene Mycoplasma genitalium antibiotic resistance doxycycline

Journal

Antimicrobial agents and chemotherapy
ISSN: 1098-6596
Titre abrégé: Antimicrob Agents Chemother
Pays: United States
ID NLM: 0315061

Informations de publication

Date de publication:
17 05 2022
Historique:
pubmed: 15 4 2022
medline: 20 5 2022
entrez: 14 4 2022
Statut: ppublish

Résumé

Doxycycline targets the 16S rRNA and is widely used for the treatment of sexually transmitted infections. While it is not highly effective at eradicating Mycoplasma genitalium infections, it can reduce organism load. The aim of this study was to investigate the association between single nucleotide polymorphisms (SNPs) in the 16S rRNA gene of M. genitalium and change in organism load. M. genitalium samples were collected from 56 men prior to commencing doxycycline and at a median of 13 of 14 doses. These were sequenced for the 16S rRNA, and the association between 16S rRNA SNPs and change in organism load was determined. 16S rRNA sequences were available for 52/56 (92.9%) M. genitalium-infected men, of which 20 (38.5%) had an undetectable load, 26 (50.0%) had a decrease in M. genitalium load (median change of 105-fold), and 6 (11.5%) had an increase in load (median change of 5-fold). The most common SNPs identified were A742G (10/52 [19.2%]), GG960-961TT/C (7/52 [13.5%]), and C1435T (28/52 [53.8%]) (M. genitalium numbering). None were associated with a change in organism load (

Identifiants

DOI: 10.1128/aac.00243-22 PMID: 35420491 PMC: PMC9112994
pubmed: 35420491
doi: 10.1128/aac.00243-22
pmc: PMC9112994
doi:

Substances chimiques

Anti-Bacterial Agents 0
RNA, Ribosomal, 16S 0
Doxycycline N12000U13O

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0024322

Références

Clin Infect Dis. 2008 Dec 15;47(12):1546-53
pubmed: 18990060
Clin Infect Dis. 2021 Oct 5;73(7):e2127-e2133
pubmed: 33305785
PLoS One. 2018 Jun 8;13(6):e0198355
pubmed: 29883482
J Clin Microbiol. 2021 Oct 19;59(11):e0081921
pubmed: 34406799
Antimicrob Agents Chemother. 1998 Jul;42(7):1702-5
pubmed: 9661007
BMC Infect Dis. 2021 Dec 7;21(1):1225
pubmed: 34876039
Clin Infect Dis. 2015 Aug 1;61(3):418-26
pubmed: 25900174
J Infect Dis. 2017 Jul 15;216(suppl_2):S396-S405
pubmed: 28838074
J Infect Chemother. 2017 Sep;23(9):648-650
pubmed: 28462860
J Infect Dis. 2017 Jul 15;216(suppl_2):S412-S419
pubmed: 28838073
Cell. 2000 Dec 22;103(7):1143-54
pubmed: 11163189
Clin Infect Dis. 2015 Dec 15;61 Suppl 8:S802-17
pubmed: 26602619
Open Forum Infect Dis. 2020 Jul 13;7(8):ofaa291
pubmed: 32782911
Clin Infect Dis. 2011 Jan 15;52(2):163-70
pubmed: 21288838
Clin Infect Dis. 2009 Jun 15;48(12):1649-54
pubmed: 19438399
Vet Microbiol. 2007 Jul 20;123(1-3):245-8
pubmed: 17428623
J Antimicrob Chemother. 2008 Jun;61(6):1390-2
pubmed: 18356155
J Bacteriol. 2005 Jun;187(11):3708-12
pubmed: 15901694
Biol Chem. 2014 May;395(5):559-75
pubmed: 24497223
Clin Infect Dis. 2013 Apr;56(7):934-42
pubmed: 23223595
EMBO J. 2001 Apr 17;20(8):1829-39
pubmed: 11296217
J Bacteriol. 2012 Nov;194(21):6010-1
pubmed: 23045512
J Bacteriol. 2002 Apr;184(8):2131-40
pubmed: 11914344
J Antimicrob Chemother. 2006 Aug;58(2):256-65
pubmed: 16816396
Antimicrob Agents Chemother. 2002 Dec;46(12):3940-6
pubmed: 12435699
Antimicrob Agents Chemother. 2002 Sep;46(9):2996-3000
pubmed: 12183259
Antimicrob Agents Chemother. 2005 Dec;49(12):4993-8
pubmed: 16304163
Clin Infect Dis. 2020 Sep 12;71(6):1461-1468
pubmed: 31629365
Emerg Infect Dis. 2018 Feb;24(2):328-335
pubmed: 29350154
Microb Drug Resist. 2011 Sep;17(3):485-8
pubmed: 21663534
Sex Transm Infect. 2008 Feb;84(1):72-6
pubmed: 17932127
J Eur Acad Dermatol Venereol. 2016 Oct;30(10):1650-1656
pubmed: 27505296
MMWR Recomm Rep. 2021 Jul 23;70(4):1-187
pubmed: 34292926
PLoS One. 2013 Apr 08;8(4):e61481
pubmed: 23593483
Int J STD AIDS. 2019 Apr;30(5):512-514
pubmed: 30999836
BMC Infect Dis. 2018 Nov 29;18(1):601
pubmed: 30486786
Antimicrob Agents Chemother. 2015 Feb;59(2):796-802
pubmed: 25403668
Antimicrob Agents Chemother. 2003 Sep;47(9):2984-6
pubmed: 12937008
Clin Infect Dis. 2019 Feb 1;68(4):554-560
pubmed: 29873691
Microb Drug Resist. 2017 Sep;23(6):799-811
pubmed: 28151090
BMC Genomics. 2017 Dec 28;18(1):993
pubmed: 29281972
Antibiotics (Basel). 2021 Aug 10;10(8):
pubmed: 34439012
Int J STD AIDS. 2019 Sep;30(10):938-950
pubmed: 31280688
Clin Infect Dis. 2022 Jan 04;:
pubmed: 34984438
Microbiol Mol Biol Rev. 2001 Jun;65(2):232-60 ; second page, table of contents
pubmed: 11381101
Antimicrob Agents Chemother. 2014 Jun;58(6):3151-6
pubmed: 24637681
J Antimicrob Chemother. 2021 Apr 13;76(5):1150-1154
pubmed: 33537731

Auteurs

Teck-Phui Chua (TP)

Molecular Microbiology Research Group, Murdoch Children's Research Institute, Parkville, Victoria, Australia.
Centre for Women's Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia.
Department of Obstetrics and Gynaecology, University of Melbournegrid.1008.9, Parkville, Victoria, Australia.

Jennifer Danielewski (J)

Molecular Microbiology Research Group, Murdoch Children's Research Institute, Parkville, Victoria, Australia.
Centre for Women's Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia.
Department of Obstetrics and Gynaecology, University of Melbournegrid.1008.9, Parkville, Victoria, Australia.

Kaveesha Bodiyabadu (K)

Molecular Microbiology Research Group, Murdoch Children's Research Institute, Parkville, Victoria, Australia.
Centre for Women's Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia.
Department of Obstetrics and Gynaecology, University of Melbournegrid.1008.9, Parkville, Victoria, Australia.

Catriona S Bradshaw (CS)

Melbourne Sexual Health Centre, Alfred Health, Carlton, Victoria, Australia.
Central Clinical School, Monash Universitygrid.1002.3, Melbourne, Victoria, Australia.

Dorothy A Machalek (DA)

Centre for Women's Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia.
The Kirby Institute, University of New South Wales, Kensington, Sydney, New South Wales, Australia.

Suzanne M Garland (SM)

Molecular Microbiology Research Group, Murdoch Children's Research Institute, Parkville, Victoria, Australia.
Centre for Women's Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia.
Department of Obstetrics and Gynaecology, University of Melbournegrid.1008.9, Parkville, Victoria, Australia.

Erica L Plummer (EL)

Molecular Microbiology Research Group, Murdoch Children's Research Institute, Parkville, Victoria, Australia.
Centre for Women's Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia.
Melbourne Sexual Health Centre, Alfred Health, Carlton, Victoria, Australia.
Central Clinical School, Monash Universitygrid.1002.3, Melbourne, Victoria, Australia.
ORCID: 0000-0001-5710-2671

Lenka A Vodstrcil (LA)

Melbourne Sexual Health Centre, Alfred Health, Carlton, Victoria, Australia.
Central Clinical School, Monash Universitygrid.1002.3, Melbourne, Victoria, Australia.

Gerald L Murray (GL)

Molecular Microbiology Research Group, Murdoch Children's Research Institute, Parkville, Victoria, Australia.
Centre for Women's Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia.
Department of Obstetrics and Gynaecology, University of Melbournegrid.1008.9, Parkville, Victoria, Australia.
ORCID: 0000-0003-3330-4433

Articles similaires

[Redispensing of expensive oral anticancer medicines: a practical application].

Lisanne N van Merendonk, Kübra Akgöl, Bastiaan Nuijen
1.00
Humans Antineoplastic Agents Administration, Oral Drug Costs Counterfeit Drugs

Smoking Cessation and Incident Cardiovascular Disease.

Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
1.00
Humans Male Smoking Cessation Cardiovascular Diseases Female

Evaluation of Low-Value Services Across Major Medicare Advantage Insurers and Traditional Medicare.

Ciara Duggan, Adam L Beckman, Ishani Ganguli et al.
1.00
Humans United States Aged Cross-Sectional Studies Medicare Part C

Effectiveness of Virtual Yoga for Chronic Low Back Pain: A Randomized Clinical Trial.

Hallie Tankha, Devyn Gaskins, Amanda Shallcross et al.
1.00
Humans Yoga Low Back Pain Female Male

Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antibactériens › Impact of 16S rRNA Single Nucleotide...
questionsmedicales.fr › Composés polycycliques › Hydrocarbures aromatiques polycycliques › Naphtacènes › Tétracyclines › Doxycycline › Impact of 16S rRNA Single Nucleotide...
questionsmedicales.fr › Phénomènes physiologiques › Phénomènes pharmacologiques et toxicologiques › Phénomènes pharmacologiques › Résistance aux substances › Résistance microbienne aux médicaments › Résistance bactérienne aux médicaments › Impact of 16S rRNA Single Nucleotide...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Impact of 16S rRNA Single Nucleotide...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram négatif › Infections à Mycoplasmatales › Infections à Mycoplasma › Impact of 16S rRNA Single Nucleotide...
questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Tenericutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium › Impact of 16S rRNA Single Nucleotide...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Polymorphisme génétique › Polymorphisme de nucléotide simple › Impact of 16S rRNA Single Nucleotide...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Collecte de données › Registre civil › Morbidité › Prévalence › Impact of 16S rRNA Single Nucleotide...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 16S › Impact of 16S rRNA Single Nucleotide...