Avian auditory hair cell regeneration is accompanied by JAK/STAT-dependent expression of immune-related genes in supporting cells.
Basilar papilla
Hair cells
Regeneration
Single cell RNA-sequencing
Supporting cells
Journal
Development (Cambridge, England)
ISSN: 1477-9129
Titre abrégé: Development
Pays: England
ID NLM: 8701744
Informations de publication
Date de publication:
15 04 2022
15 04 2022
Historique:
received:
23
09
2021
accepted:
31
03
2022
pubmed:
15
4
2022
medline:
6
5
2022
entrez:
14
4
2022
Statut:
ppublish
Résumé
The avian hearing organ is the basilar papilla that, in sharp contrast to the mammalian cochlea, can regenerate sensory hair cells and thereby recover from deafness within weeks. The mechanisms that trigger, sustain and terminate the regenerative response in vivo are largely unknown. Here, we profile the changes in gene expression in the chicken basilar papilla after aminoglycoside antibiotic-induced hair cell loss using RNA-sequencing. We identified changes in gene expression of a group of immune-related genes and confirmed with single-cell RNA-sequencing that these changes occur in supporting cells. In situ hybridization was used to further validate these findings. We determined that the JAK/STAT signaling pathway is essential for upregulation of the damage-response genes in supporting cells during the second day after induction of hair cell loss. Four days after ototoxic damage, we identified newly regenerated, nascent auditory hair cells that express genes linked to termination of the JAK/STAT signaling response. The robust, transient expression of immune-related genes in supporting cells suggests a potential functional involvement of JAK/STAT signaling in sensory hair cell regeneration.
Identifiants
pubmed: 35420675
pii: 275061
doi: 10.1242/dev.200113
pmc: PMC10656459
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
RNA
63231-63-0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIDCD NIH HHS
ID : R01 DC019370
Pays : United States
Organisme : NIDCD NIH HHS
ID : R01 DC019619
Pays : United States
Organisme : NIDCD NIH HHS
ID : U01 DC019370
Pays : United States
Informations de copyright
© 2022. Published by The Company of Biologists Ltd.
Déclaration de conflit d'intérêts
Competing interests S.H. is a paid scientific advisor of Pipeline Therapeutics.
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