Effects of Antifreeze Protein Type I and Glycerol in Diluents on Cryopreserved Goat Epididymal Sperm.
antifreeze protein
buck sperm
cryopreservation
epididymal spermatozoa
glycerol
Journal
Biopreservation and biobanking
ISSN: 1947-5543
Titre abrégé: Biopreserv Biobank
Pays: United States
ID NLM: 101507284
Informations de publication
Date de publication:
Feb 2023
Feb 2023
Historique:
pubmed:
16
4
2022
medline:
22
2
2023
entrez:
15
4
2022
Statut:
ppublish
Résumé
The effect of antifreeze protein (AFP) as a cryoprotectant used in different concentrations of glycerol on post-thaw quality of epididymal sperm was investigated. Sperm were isolated from 50 testicles, obtained from 25 healthy mature goat bucks, with progressive motility >80%, and total morphological abnormalities <10% were pooled in each replication. The semen samples were diluted with Tris-citrate-fructose-soybean lecithin extender containing different concentration of AFP [0 μg/mL (A0), 5 μg/mL (A5), 10 μg/mL (A10)]. Each concentration of AFP was added in an extender containing either 7% (G7) or 5% (G5) glycerol. Post-thaw total and progressive motility were found to be higher (
Identifiants
pubmed: 35426727
doi: 10.1089/bio.2021.0150
doi:
Substances chimiques
Glycerol
PDC6A3C0OX
alpha-Fetoproteins
0
Cryoprotective Agents
0
Antifreeze Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM