Berberine Modulates Macrophage Activation by Inducing Glycolysis.
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
15 05 2022
15 05 2022
Historique:
received:
19
07
2021
accepted:
28
02
2022
pubmed:
17
4
2022
medline:
20
5
2022
entrez:
16
4
2022
Statut:
ppublish
Résumé
Classical activation of macrophage and monocyte differentiation induced by β-glucan is accompanied with metabolic change in glucose. However, the role of the metabolic rewiring in monocyte/macrophage activation remains elusive. In this study, we show that berberine induces aerobic glycolysis by blocking the tricarboxylic acid cycle and modulates cytokine responses in bone marrow-derived macrophages (BMDMs) from mice and human PBMC. 13-Methyberberine had activities on glucose metabolism and BMDM activation similar to those of berberine, whereas other tested derivatives lost both activities. Glucose transporter (GLUT)1 expression and total cellular hexokinase activity increased gradually in BMDMs in the presence of berberine. In the contrast, LPS upregulated GLUT1 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) levels in 6 h. Extracellular glucose levels and replacing glucose with galactose in culture medium affected the cytokine secretion of BMDMs. Berberine alleviated enteritis of
Identifiants
pubmed: 35428692
pii: jimmunol.2100716
doi: 10.4049/jimmunol.2100716
doi:
Substances chimiques
Lipopolysaccharides
0
Tumor Necrosis Factor-alpha
0
Berberine
0I8Y3P32UF
Phosphofructokinase-2
EC 2.7.1.105
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2309-2318Informations de copyright
Copyright © 2022 by The American Association of Immunologists, Inc.