Intracellular pH affects mitochondrial homeostasis in cultured human corneal endothelial cells prepared for cell injection therapy.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 04 2022
15 04 2022
Historique:
received:
10
11
2021
accepted:
30
03
2022
pubmed:
17
4
2022
medline:
20
4
2022
entrez:
16
4
2022
Statut:
epublish
Résumé
This study aimed to uncover the mechanism responsible for the clinical efficacy of cell injection therapy with fully differentiated cultured cells. Analysis of polarized expression of ion transporters on cultured human corneal endothelial cells (CECs) subpopulations (SPs) was performed. The intracellular pH (pHi) between two CEC SPs, distinct in the proportion of differentiated cells, was measured, and the association with mitochondrial respiration homeostasis was investigated. The effects of the ion transporter inhibition by their selective inhibitors or siRNA transfection were also explored. Na
Identifiants
pubmed: 35428816
doi: 10.1038/s41598-022-10176-1
pii: 10.1038/s41598-022-10176-1
pmc: PMC9012833
doi:
Substances chimiques
Anion Transport Proteins
0
Antiporters
0
RNA, Small Interfering
0
SLC4A11 protein, human
0
Water
059QF0KO0R
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6263Informations de copyright
© 2022. The Author(s).
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