A Butyrylcholinesterase Camera Biosensor Tested for Carbofuran and Paraoxon Assay.
Journal
International journal of analytical chemistry
ISSN: 1687-8760
Titre abrégé: Int J Anal Chem
Pays: Egypt
ID NLM: 101519424
Informations de publication
Date de publication:
2022
2022
Historique:
received:
29
01
2022
accepted:
28
03
2022
entrez:
18
4
2022
pubmed:
19
4
2022
medline:
19
4
2022
Statut:
epublish
Résumé
Biosensors containing cholinesterase are analytical devices suitable for the assay of neurotoxic compounds. In the research on biosensors, a new platform has appeared some years ago. It is the digital photography and scoring of coloration (photogrammetry). In this paper, a colorimetric biosensor is constructed using 3D-printed multiwell pads treated with indoxylacetate as a chromogenic substrate and gold nanoparticles with the immobilized enzyme butyrylcholinesterase. A smartphone camera served for photogrammetry. The biosensor was tested for the assay of carbofuran and paraoxon ethyl as two types of covalently binding inhibitors: irreversible and pseudoirreversible. The biosensor exerted good sensitivity to the inhibitors and was able to detect carbofuran with a limit of detection for carbofuran 7.7 nmol/l and 17.6 nmol/l for paraoxon ethyl. A sample sized 25
Identifiants
pubmed: 35432544
doi: 10.1155/2022/2623155
pmc: PMC9010193
doi:
Types de publication
Journal Article
Langues
eng
Pagination
2623155Informations de copyright
Copyright © 2022 Miroslav Pohanka and Jitka Zakova.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
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