A Rapid, Highly Sensitive and Open-Access SARS-CoV-2 Detection Assay for Laboratory and Home Testing.
COVID-19 diagnostics
RT-LAMP
SARS-CoV-2
coronavirus
isothermal amplification
open-source
Journal
Frontiers in molecular biosciences
ISSN: 2296-889X
Titre abrégé: Front Mol Biosci
Pays: Switzerland
ID NLM: 101653173
Informations de publication
Date de publication:
2022
2022
Historique:
received:
25
10
2021
accepted:
01
03
2022
entrez:
18
4
2022
pubmed:
19
4
2022
medline:
19
4
2022
Statut:
epublish
Résumé
RT-qPCR-based diagnostic tests play important roles in combating virus-caused pandemics such as Covid-19. However, their dependence on sophisticated equipment and the associated costs often limits their widespread use. Loop-mediated isothermal amplification after reverse transcription (RT-LAMP) is an alternative nucleic acid detection method that overcomes these limitations. Here, we present a rapid, robust, and sensitive RT-LAMP-based SARS-CoV-2 detection assay. Our 40-min procedure bypasses the RNA isolation step, is insensitive to carryover contamination, and uses a colorimetric readout that enables robust SARS-CoV-2 detection from various sample types. Based on this assay, we have increased sensitivity and scalability by adding a nucleic acid enrichment step (Bead-LAMP), developed a version for home testing (HomeDip-LAMP), and identified open-source RT-LAMP enzymes that can be produced in any molecular biology laboratory. On a dedicated website, rtlamp.org (DOI: 10.5281/zenodo.6033689), we provide detailed protocols and videos. Our optimized, general-purpose RT-LAMP assay is an important step toward population-scale SARS-CoV-2 testing.
Identifiants
pubmed: 35433827
doi: 10.3389/fmolb.2022.801309
pii: 801309
pmc: PMC9011764
doi:
Types de publication
Journal Article
Langues
eng
Pagination
801309Informations de copyright
Copyright © 2022 Kellner, Ross, Schnabl, Dekens, Matl, Heinen, Grishkovskaya, Bauer, Stadlmann, Menéndez-Arias, Straw, Fritsche-Polanz, Traugott, Seitz, Zoufaly, Födinger, Wenisch, Zuber, Vienna COVID-19 Detection Initiative (VCDI), Pauli and Brennecke.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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