The Potential Risk of International Spread of
Enferplex Camelid TB test
Mycobacterium bovis
alpaca
tuberculin skin test
Journal
Journal of veterinary research
ISSN: 2450-7393
Titre abrégé: J Vet Res
Pays: Poland
ID NLM: 101696630
Informations de publication
Date de publication:
Mar 2022
Mar 2022
Historique:
received:
12
01
2022
accepted:
01
03
2022
entrez:
18
4
2022
pubmed:
19
4
2022
medline:
19
4
2022
Statut:
epublish
Résumé
The study highlights the transboundary nature of tuberculosis (TB) in alpacas and the failure of current ante-mortem testing protocols (the tuberculin skin and Enferplex Camelid TB tests) to identify TB-free alpaca herds and individuals for export. Our research and the available literature indicate that the alpaca ( Thoracic lymph node, lung and liver tissue samples obtained at necropsy were examined histopathologically after Ziehl-Neelsen staining. Tissue samples were homogenised and mycobacteria present there were cultured on Stonebrink's medium during a 6-week incubation. A commercial test using polymorphism of the chromosomal direct repeat region provided species identification and additional identification was by spacer oligonucleotide typing and mycobacteria interspersed repetitive unit-variable number tandem repeat analysis with a gel electrophoresis protocol. The microbiological examination confirmed multiorgan TB caused by the SB0666 spoligotype of Due to the suboptimal performance of current diagnostic tests for TB in alpacas, there is a risk that infected animals may be moved unwittingly. A risk of TB spread associated with the international movement of alpacas is implied by this study.
Identifiants
pubmed: 35434415
doi: 10.2478/jvetres-2022-0012
pii: jvetres-2022-0012
pmc: PMC8959691
doi:
Types de publication
Journal Article
Langues
eng
Pagination
53-59Informations de copyright
© 2022 M. Krajewska-Wędzina et al. published by Sciendo.
Déclaration de conflit d'intérêts
Conflict of Interest Conflict of Interests Statement: The authors declare that there is no conflict of interests regarding the publication of this article.
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