Dithiothreitol causes toxicity in
C. elegans
ER stress
S-adenosylmethionine
biochemistry
chemical biology
dithiothreitol
methionine cycle
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
19 04 2022
19 04 2022
Historique:
received:
01
12
2021
accepted:
17
04
2022
pubmed:
20
4
2022
medline:
14
5
2022
entrez:
19
4
2022
Statut:
epublish
Résumé
The redox reagent dithiothreitol (DTT) causes stress in the endoplasmic reticulum (ER) by disrupting its oxidative protein folding environment, which results in the accumulation and misfolding of the newly synthesized proteins. DTT may potentially impact cellular physiology by ER-independent mechanisms; however, such mechanisms remain poorly characterized. Using the nematode model Animal and plant cells synthesize a significant fraction of their proteins on a structure known as the endoplasmic reticulum. Researchers often use the molecule dithiothreitol to specifically target this compartment and learn more about its role. The toxin works by disturbing the complex chemical environment present in the reticulum, which is required for the proteins to assemble properly. However, it is important to clarify whether dithiothreitol could also affect other parts of the cell, as this could give rise to misleading results. To explore this possibility, Gokul G and Jogender Singh studied the effects of dithiothreitol on the millimetre-long roundworm
Autres résumés
Type: plain-language-summary
(eng)
Animal and plant cells synthesize a significant fraction of their proteins on a structure known as the endoplasmic reticulum. Researchers often use the molecule dithiothreitol to specifically target this compartment and learn more about its role. The toxin works by disturbing the complex chemical environment present in the reticulum, which is required for the proteins to assemble properly. However, it is important to clarify whether dithiothreitol could also affect other parts of the cell, as this could give rise to misleading results. To explore this possibility, Gokul G and Jogender Singh studied the effects of dithiothreitol on the millimetre-long roundworm
Identifiants
pubmed: 35438636
doi: 10.7554/eLife.76021
pii: 76021
pmc: PMC9090326
doi:
pii:
Substances chimiques
Caenorhabditis elegans Proteins
0
Homocysteine
0LVT1QZ0BA
S-Adenosylmethionine
7LP2MPO46S
Dithiothreitol
T8ID5YZU6Y
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIH HHS
ID : P40 OD010440
Pays : United States
Informations de copyright
© 2022, G and Singh.
Déclaration de conflit d'intérêts
GG, JS No competing interests declared
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