Hyperactivation is sufficient to release porcine sperm from immobilized oviduct glycans.

Animals Female Humans Male Oviducts / metabolism Polysaccharides / metabolism Sperm Capacitation / physiology Sperm Motility / physiology Spermatozoa / metabolism Swine

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
19 04 2022
Historique:
received: 14 09 2021
accepted: 14 03 2022
entrez: 20 4 2022
pubmed: 21 4 2022
medline: 22 4 2022
Statut: epublish

Résumé

Fertilizing sperm are retained by adhesion to specific glycans on the epithelium of the oviduct forming a reservoir before sperm are released from the reservoir so fertilization can ensue. Capacitated sperm lose affinity for the oviduct epithelium but the components of capacitation that are important for sperm release are uncertain. One important correlate of capacitation is the development of hyperactivated motility. Hyperactivation is characterized by asymmetrical flagellar beating with high beat amplitude. We tested whether the development of full-type asymmetrical motility was sufficient to release sperm from immobilized oviduct glycans. Sperm hyperactivation was induced by four different compounds, a cell-permeable cAMP analog (cBiMPS), CatSper activators (4-aminopyridine and procaine), and an endogenous steroid (progesterone). Using standard analysis (CASA) and direct visualization with high-speed video microscopy, we first confirmed that all four compounds induced hyperactivation. Subsequently, sperm were allowed to bind to immobilized oviduct glycans, and compounds or vehicle controls were added. All compounds caused sperm release from immobilized glycans, demonstrating that hyperactivation was sufficient to release sperm from oviduct cells and immobilized glycans. Pharmacological inhibition of the non-genomic progesterone receptor and CatSper diminished sperm release from oviduct glycans. Inhibition of the proteolytic activities of the ubiquitin-proteasome system (UPS), implicated in the regulation of sperm capacitation, diminished sperm release in response to all hyperactivation inducers. In summary, induction of sperm hyperactivation was sufficient to induce sperm release from immobilized oviduct glycans and release was dependent on CatSper and the UPS.

Identifiants

DOI: 10.1038/s41598-022-10390-x PMID: 35440797 PMC: PMC9019019
pubmed: 35440797
doi: 10.1038/s41598-022-10390-x
pii: 10.1038/s41598-022-10390-x
pmc: PMC9019019
doi:

Substances chimiques

Polysaccharides 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

6446

Subventions

Organisme : NICHD NIH HHS
ID : R01 HD095841
Pays : United States
Organisme : NIH HHS
ID : RO1HD095841
Pays : United States

Informations de copyright

© 2022. The Author(s).

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Auteurs

Momal Sharif (M)

Department of Animal Sciences and Institute of Genomic Biology, University of Illinois at Urbana-Champaign, 1207 West Gregory Drive, Urbana, IL, 61801, USA.
Department of Obstetrics and Gynecology, Baylor College of Medicine and Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Houston, TX, 77030, USA.

Vincent Hickl (V)

Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

Gabriel Juarez (G)

Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

Xingjian Di (X)

Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL, 61801, USA.

Karl Kerns (K)

Department of Animal Science, Iowa State University, Ames, IA, 50011, USA.
Division of Animal Sciences, University of Missouri, Columbia, MO, 65211, USA.

Peter Sutovsky (P)

Division of Animal Sciences, University of Missouri, Columbia, MO, 65211, USA.
Department of Obstetrics, Gynecology and Women's Health, University of Missouri, Columbia, MO, 65211, USA.

Nicolai Bovin (N)

Shemyakin Institute of Bioorganic Chemistry, Moscow, Russia.

David J Miller (DJ)

Department of Animal Sciences and Institute of Genomic Biology, University of Illinois at Urbana-Champaign, 1207 West Gregory Drive, Urbana, IL, 61801, USA. djmille@illinois.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Hyperactivation is sufficient to release...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Hyperactivation is sufficient to release...
questionsmedicales.fr › Structures anatomiques de l'animal › Oviductes › Hyperactivation is sufficient to release...
questionsmedicales.fr › Glucides › Polyosides › Hyperactivation is sufficient to release...
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Fécondation › Capacitation des spermatozoïdes › Hyperactivation is sufficient to release...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Mobilité des spermatozoïdes › Hyperactivation is sufficient to release...
questionsmedicales.fr › Cellules › Cellules germinales › Spermatozoïdes › Hyperactivation is sufficient to release...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Suidae › Hyperactivation is sufficient to release...