Urinary interleukin-9 in youth with type 1 diabetes mellitus.
Adolescent
Albuminuria
/ urine
Biomarkers
/ urine
Creatinine
/ urine
Cytokines
/ urine
Diabetes Mellitus, Type 1
/ urine
Diabetic Nephropathies
/ urine
Glycated Hemoglobin
Humans
Interleukin-6
/ urine
Interleukin-9
/ urine
Tumor Necrosis Factor-alpha
/ urine
Vascular Endothelial Growth Factor A
/ urine
Albuminuria
Cytokines
Extracellular vesicle
IL9
Podocyte
Type 1 diabetes mellitus
Journal
Acta diabetologica
ISSN: 1432-5233
Titre abrégé: Acta Diabetol
Pays: Germany
ID NLM: 9200299
Informations de publication
Date de publication:
Jul 2022
Jul 2022
Historique:
received:
18
10
2021
accepted:
20
02
2022
pubmed:
22
4
2022
medline:
3
6
2022
entrez:
21
4
2022
Statut:
ppublish
Résumé
Interleukin-9 (IL-9) attenuates podocyte injury in experimental kidney disease, but its role in diabetic nephropathy is unknown. We sought to relate urinary IL-9 levels to the release of podocyte-derived extracellular vesicles (EVs) in youth with type 1 diabetes. We related urinary IL-9 levels to clinical variables and studied interactions between urinary IL-9, vascular endothelial growth factor (VEGF), tumor necrosis factor alpha (TNFα) and interleukin-6 (IL-6) on urinary albumin/creatinine ratio (ACR) a functional measure of podocyte injury. We performed an analysis of urine samples and clinical data from a cohort of youth with type 1 diabetes (n = 53). Cytokines were measured using a Luminex platform (Eve Technologies), and nanoscale flow cytometry was employed to quantify urinary podocyte-derived EVs. All urinary measures were normalized to urinary creatinine. Mean age was 14.7 ± 1.6 years, and the mean time from diagnosis was 6.7 ± 2.9 years. Mean HbA1c was 70.3 ± 13.9 mmol/mol, mean ACR was 1.3 ± 1.9 mg/mmol, and mean eGFR was 140.3 ± 32.6 ml/min/1.73 m IL-9 is associated with podocyte injury in early type 1 diabetes, and there are complex interactions between urinary IL-9, inflammatory cytokines and ACR.
Identifiants
pubmed: 35445345
doi: 10.1007/s00592-022-01873-4
pii: 10.1007/s00592-022-01873-4
pmc: PMC9156513
doi:
Substances chimiques
Biomarkers
0
Cytokines
0
Glycated Hemoglobin A
0
IL9 protein, human
0
Interleukin-6
0
Interleukin-9
0
Tumor Necrosis Factor-alpha
0
Vascular Endothelial Growth Factor A
0
Creatinine
AYI8EX34EU
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
939-947Subventions
Organisme : CIHR
ID : MOP-343810
Pays : Canada
Organisme : CIHR
ID : MOP-343810
Pays : Canada
Informations de copyright
© 2022. The Author(s).
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