A Folding-Based Electrochemical Aptasensor for the Single-Step Detection of the SARS-CoV-2 Spike Protein.
COVID-19
DNA aptamer
DNA nanotechnology
electrochemical sensors
single-walled carbon nanotubes
Journal
ACS applied materials & interfaces
ISSN: 1944-8252
Titre abrégé: ACS Appl Mater Interfaces
Pays: United States
ID NLM: 101504991
Informations de publication
Date de publication:
04 May 2022
04 May 2022
Historique:
pubmed:
22
4
2022
medline:
7
5
2022
entrez:
21
4
2022
Statut:
ppublish
Résumé
Efficient and timely testing has taken center stage in the management, control, and monitoring of the current COVID-19 pandemic. Simple, rapid, cost-effective diagnostics are needed that can complement current polymerase chain reaction-based methods and lateral flow immunoassays. Here, we report the development of an electrochemical sensing platform based on single-walled carbon nanotube screen-printed electrodes (SWCNT-SPEs) functionalized with a redox-tagged DNA aptamer that specifically binds to the receptor binding domain of the SARS-CoV-2 spike protein S1 subunit. Single-step, reagentless detection of the S1 protein is achieved through a binding-induced, concentration-dependent folding of the DNA aptamer that reduces the efficiency of the electron transfer process between the redox tag and the electrode surface and causes a suppression of the resulting amperometric signal. This aptasensor is specific for the target S1 protein with a dissociation constant (
Identifiants
pubmed: 35446532
doi: 10.1021/acsami.2c02405
pmc: PMC9045037
doi:
Substances chimiques
Aptamers, Nucleotide
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
19204-19211Références
J Am Chem Soc. 2021 Feb 3;143(4):1722-1727
pubmed: 33481575
Sens Actuators B Chem. 2021 Oct 15;345:130377
pubmed: 34219971
Bioelectrochemistry. 2022 Feb;143:107982
pubmed: 34715586
Lancet. 2021 May 15;397(10287):1783-1785
pubmed: 33964221
Microchem J. 2021 Nov;170:106718
pubmed: 34381282
Nat Rev Microbiol. 2021 Mar;19(3):141-154
pubmed: 33024307
Biosens Bioelectron. 2008 Mar 14;23(8):1291-7
pubmed: 18178423
Lancet Infect Dis. 2021 Aug;21(8):1052-1053
pubmed: 33773617
Nano Lett. 2021 Mar 10;21(5):2272-2280
pubmed: 33635655
Acc Chem Res. 2010 Apr 20;43(4):496-505
pubmed: 20201486
Biosens Bioelectron. 2019 Mar 15;129:7-14
pubmed: 30682690
Best Pract Res Clin Anaesthesiol. 2021 Oct;35(3):293-306
pubmed: 34511220
N Engl J Med. 2021 Dec 9;385(24):e83
pubmed: 34614327
Trends Analyt Chem. 2021 Dec;145:116452
pubmed: 34629572
Acc Chem Res. 2010 May 18;43(5):631-41
pubmed: 20222738
Biosens Bioelectron. 2021 Jan 1;171:112686
pubmed: 33086175
Anal Chem. 2022 Mar 29;94(12):5075-5083
pubmed: 35303407
Angew Chem Int Ed Engl. 2021 Apr 26;60(18):10266-10272
pubmed: 33561300
Mater Lett. 2022 Feb 1;308:131237
pubmed: 34776564
Mikrochim Acta. 2021 Mar 2;188(3):105
pubmed: 33651173
ACS Omega. 2021 Dec 15;6(51):35657-35666
pubmed: 34957366
Sensors (Basel). 2019 Jan 30;19(3):
pubmed: 30704111
Sci Adv. 2021 Sep 24;7(39):eabh2848
pubmed: 34550739
Bioelectrochemistry. 2007 Jan;70(1):127-33
pubmed: 16725379
Anal Chem. 2007 Feb 15;79(4):1466-73
pubmed: 17297945
Nat Protoc. 2016 Jul;11(7):1244-63
pubmed: 27310264
ACS Sens. 2021 Sep 24;6(9):3340-3347
pubmed: 34491055
ACS Sens. 2021 Aug 27;6(8):3093-3101
pubmed: 34375076
Biosens Bioelectron. 2020 Sep 1;163:112274
pubmed: 32421627
Trends Analyt Chem. 2020 Sep;130:115984
pubmed: 32834243
ACS Appl Mater Interfaces. 2021 May 19;13(19):22262-22270
pubmed: 33966371
Chem Rev. 2016 Aug 24;116(16):9001-90
pubmed: 27428515
ACS Nano. 2020 Apr 28;14(4):5135-5142
pubmed: 32293168
Sensors (Basel). 2020 Apr 03;20(7):
pubmed: 32260240
Sens Actuators B Chem. 2022 Mar 15;355:131324
pubmed: 34987275
J Am Chem Soc. 2012 Feb 22;134(7):3346-8
pubmed: 22313286
Angew Chem Int Ed Engl. 2005 Aug 26;44(34):5456-9
pubmed: 16044476
N Engl J Med. 2021 Nov 4;385(19):1761-1773
pubmed: 34525277
Anal Chem. 2020 Jul 21;92(14):9895-9900
pubmed: 32551560
Anal Methods. 2021 Nov 25;13(45):5400-5417
pubmed: 34751684
Anal Chem. 2021 Jan 26;93(3):1826-1833
pubmed: 33370087
J Am Soc Mass Spectrom. 2017 Sep;28(9):1815-1822
pubmed: 28755259
Anal Chem. 2017 Nov 21;89(22):12185-12191
pubmed: 29076341
Nucleic Acids Res. 2021 Jul 21;49(13):7267-7279
pubmed: 34232998
Angew Chem Int Ed Engl. 2020 Nov 9;59(46):20577-20581
pubmed: 32737920
Biosens Bioelectron. 2021 Mar 15;176:112905
pubmed: 33358285
Angew Chem Int Ed Engl. 2021 Nov 2;60(45):24266-24274
pubmed: 34464491
Trends Biochem Sci. 2020 Aug;45(8):639-649
pubmed: 32402748
Anal Chem. 2007 Jan 1;79(1):229-33
pubmed: 17194144
Nature. 2020 Aug;584(7821):425-429
pubmed: 32604404
ACS Nano. 2021 Oct 27;:
pubmed: 34705433
Angew Chem Int Ed Engl. 2021 Sep 20;60(39):21211-21215
pubmed: 34328683
Biosens Bioelectron. 2017 Nov 15;97:226-237
pubmed: 28601788