Molecular Diagnosis of Two Major Implantation Mycoses: Chromoblastomycosis and Sporotrichosis.

MALDI-TOF MS PCR chromoblastomycosis isothermal PCR molecular sequencing sporotrichosis

Journal

Journal of fungi (Basel, Switzerland)
ISSN: 2309-608X
Titre abrégé: J Fungi (Basel)
Pays: Switzerland
ID NLM: 101671827

Informations de publication

Date de publication:
09 Apr 2022
Historique:
received: 11 03 2022
revised: 04 04 2022
accepted: 06 04 2022
entrez: 21 4 2022
pubmed: 22 4 2022
medline: 22 4 2022
Statut: epublish

Résumé

Chromoblastomycosis and sporotrichosis are the two main implantation mycoses that are now recognized as fungal neglected tropical diseases (NTDs). Their laboratory diagnosis mainly relies on direct microscopy, histopathology, and identification of the fungus by culture. However, to be appropriately used, these techniques require mycological expertise that is not widely available and may be absent in peripheral health care facilities in endemic areas. In addition, they lack sensitivity and specificity, and the culture for isolation and identification can have a long time-to-results period. Molecular methods, including matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), have been developed in well-equipped reference laboratories. They greatly improve the rapidity and accuracy of diagnosis; in particular, for species identification. Recently, PCR and sequencing have paved the way for more user-friendly point-of-care tests, such as those based on LAMP or RCA technologies, which can be used in basic healthcare settings and even in field consultations.

Identifiants

pubmed: 35448613
pii: jof8040382
doi: 10.3390/jof8040382
pmc: PMC9027143
pii:
doi:

Types de publication

Journal Article Review

Langues

eng

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Auteurs

Danièle Maubon (D)

Translational Innovation in Medicine and Complexity, Centre National de la Recherche Scientifique, Université Grenoble Alpes, Domaine de la Merci, Centre Hospitalier Universitaire Grenoble Alpes, Service de Parasitologie-Mycologie, Bd de la Chantourne, CEDEX, 38706 La Tronche, France.

Cécile Garnaud (C)

Translational Innovation in Medicine and Complexity, Centre National de la Recherche Scientifique, Université Grenoble Alpes, Domaine de la Merci, Centre Hospitalier Universitaire Grenoble Alpes, Service de Parasitologie-Mycologie, Bd de la Chantourne, CEDEX, 38706 La Tronche, France.

Lala Soavina Ramarozatovo (LS)

Université Hospital Joseph Raseta Befelatanana, Antananarivo 101, Madagascar.

Rapelanoro Rabenja Fahafahantsoa (RR)

Université Hospital Joseph Raseta Befelatanana, Antananarivo 101, Madagascar.

Muriel Cornet (M)

Translational Innovation in Medicine and Complexity, Centre National de la Recherche Scientifique, Université Grenoble Alpes, Domaine de la Merci, Centre Hospitalier Universitaire Grenoble Alpes, Service de Parasitologie-Mycologie, Bd de la Chantourne, CEDEX, 38706 La Tronche, France.

Tahinamandranto Rasamoelina (T)

Centre d'Infectiologie Charles Mérieux, Université d'Antananarivo, Antananarivo 101, Madagascar.

Classifications MeSH