Laponite/amoxicillin-functionalized PLA nanofibrous as osteoinductive and antibacterial scaffolds.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
21 04 2022
21 04 2022
Historique:
received:
25
11
2021
accepted:
11
04
2022
entrez:
22
4
2022
pubmed:
23
4
2022
medline:
26
4
2022
Statut:
epublish
Résumé
In this study, Amoxicillin (AMX) was loaded on laponite (LAP) nanoplates and then immobilized on the surface of electrospun polylactic acid (PLA) nanofibers to fabricate scaffolds with osteoinductive and antibacterial activities. The highest loading efficiency (49%) was obtained when the concentrations of AMX and LAP were 3 mg/mL and 1 mg/mL, respectively. FTIR and XRD spectroscopies and zeta potentiometry confirmed the successful encapsulating of AMX within LAP nanoplates. The immobilization of AMX-loaded LAPs on the surface of PLA nanofibers was verified by SEM and FTIR spectroscopy. In vitro release study showed a two-phase AMX release profile for the scaffolds; an initial burst release within the first 48 h and a later sustained release up to 21 days. In vitro antibacterial tests against Staphylococcus aureus and Escherichia coli presented the ability of scaffolds to inhibit the growth of both bacteria. The biocompatibility assays revealed the attachment and viability of human bone marrow mesenchymal stem cells (hBMSCs) cultured on the surface of scaffolds (p ≤ 0.05). The increased ALKALINE PHOSPHATASE (ALP) activity (p ≤ 0.001), calcium deposition, and expression of ALP and OSTEONECTIN genes indicated the osteoinductivity of functionalized scaffolds for hBMSCs. These LAP/AMX-functionalized scaffolds might be desirable candida for the treatment of bone defects.
Identifiants
pubmed: 35449188
doi: 10.1038/s41598-022-10595-0
pii: 10.1038/s41598-022-10595-0
pmc: PMC9023499
doi:
Substances chimiques
Anti-Bacterial Agents
0
Polyesters
0
Silicates
0
poly(lactide)
459TN2L5F5
Amoxicillin
804826J2HU
laponite
D703131383
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6583Informations de copyright
© 2022. The Author(s).
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