Retinal Microvasculature-on-a-Chip for Modeling VEGF-Induced Permeability.
3D model
Advanced in vitro model
Blood-retinal barrier
High content imaging
Microvasculature
Organ-on-a-chip
VEGF-induced leakage
Vascular permeability
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
22
4
2022
pubmed:
23
4
2022
medline:
27
4
2022
Statut:
ppublish
Résumé
Relevant human in vitro models of the retinal microvasculature can be used to study the role of disease mediators on retinal barrier dysfunction and assess the efficacy of early drug candidates. This chapter describes an organ-on-a-chip model of the retinal microvasculature that allows for facile quantification of barrier permeability in response to leakage mediators, such as Vascular Endothelial Growth Factor (VEGF), and enables screening of VEGF-induced permeability inhibitors. This chapter also presents an automated confocal imaging method for the visualization of endothelial tube morphology as an additional measure of barrier integrity.
Identifiants
pubmed: 35451763
doi: 10.1007/978-1-0716-2217-9_18
doi:
Substances chimiques
Vascular Endothelial Growth Factor A
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
239-257Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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