Elucidating miRNA Function in Cancer Biology via the Molecular Genetics' Toolbox.
CRISPR/Cas9
antagomir
dCas9 modifications
epigenetics
miRNA mimic
miRNASponges
microRNA
Journal
Biomedicines
ISSN: 2227-9059
Titre abrégé: Biomedicines
Pays: Switzerland
ID NLM: 101691304
Informations de publication
Date de publication:
15 Apr 2022
15 Apr 2022
Historique:
received:
25
10
2021
revised:
23
11
2021
accepted:
30
11
2021
entrez:
23
4
2022
pubmed:
24
4
2022
medline:
24
4
2022
Statut:
epublish
Résumé
Micro-RNA (miRNAs) are short non-coding RNAs of about 18-20 nucleotides in length and are implicated in many cellular processes including proliferation, development, differentiation, apoptosis and cell signaling. Furthermore, it is well known that miRNA expression is frequently dysregulated in many cancers. Therefore, this review will highlight the various mechanisms by which microRNAs are dysregulated in cancer. Further highlights include the abundance of molecular genetics tools that are currently available to study miRNA function as well as their advantages and disadvantages with a special focus on various CRISPR/Cas systems This review provides general workflows and some practical considerations when studying miRNA function thus enabling researchers to make informed decisions in regards to the appropriate molecular genetics tool to be utilized for their experiments.
Identifiants
pubmed: 35453665
pii: biomedicines10040915
doi: 10.3390/biomedicines10040915
pmc: PMC9029477
pii:
doi:
Types de publication
Journal Article
Review
Langues
eng
Subventions
Organisme : Ministry of Higher Education
ID : FRGS/1/2018/SKK08/USM/02/8 (203/CIPPT/6711672)
Organisme : Universiti Sains Malaysia
ID : USM-RUI: 1001/CIPPT/8012265
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