Single-Cell Sequencing: Ariadne's Thread in the Maze of Acute Myeloid Leukemia.

acute myeloid leukemia clonal evolution clonal heterogeneity single-cell DNA sequencing single-cell RNA sequencing

Journal

Diagnostics (Basel, Switzerland)
ISSN: 2075-4418
Titre abrégé: Diagnostics (Basel)
Pays: Switzerland
ID NLM: 101658402

Informations de publication

Date de publication:
15 Apr 2022
Historique:
received: 07 03 2022
revised: 12 04 2022
accepted: 14 04 2022
entrez: 23 4 2022
pubmed: 24 4 2022
medline: 24 4 2022
Statut: epublish

Résumé

Acute myeloid leukemia (AML) is a haematological neoplasm resulting from the accumulation of genetic and epigenetic alterations. Patients' prognoses vary with AML genetic heterogeneity, which hampers successful treatments. Single-cell approaches have provided new insights of the clonal architecture of AML, revealing the mutational history from diagnosis, during treatment and to relapse. In this review, we imagine single-cell technologies as the Ariadne's thread that will guide us out of the AML maze, provide a precise identikit of the leukemic cell at single-cell resolution and explore genomic, transcriptomic, epigenetic and proteomic levels.

Identifiants

pubmed: 35454044
pii: diagnostics12040996
doi: 10.3390/diagnostics12040996
pmc: PMC9024495
pii:
doi:

Types de publication

Journal Article Review

Langues

eng

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Auteurs

Immacolata Redavid (I)

Hematology Section, Department of Emergency and Organ Transplantation (D.E.T.O.), University of Bari 'Aldo Moro', 70124 Bari, Italy.

Maria Rosa Conserva (MR)

Hematology Section, Department of Emergency and Organ Transplantation (D.E.T.O.), University of Bari 'Aldo Moro', 70124 Bari, Italy.

Luisa Anelli (L)

Hematology Section, Department of Emergency and Organ Transplantation (D.E.T.O.), University of Bari 'Aldo Moro', 70124 Bari, Italy.

Antonella Zagaria (A)

Hematology Section, Department of Emergency and Organ Transplantation (D.E.T.O.), University of Bari 'Aldo Moro', 70124 Bari, Italy.

Giorgina Specchia (G)

School of Medicine, University of Bari 'Aldo Moro', 70124 Bari, Italy.

Pellegrino Musto (P)

Hematology Section, Department of Emergency and Organ Transplantation (D.E.T.O.), University of Bari 'Aldo Moro', 70124 Bari, Italy.

Francesco Albano (F)

Hematology Section, Department of Emergency and Organ Transplantation (D.E.T.O.), University of Bari 'Aldo Moro', 70124 Bari, Italy.

Classifications MeSH