Continuous Percoll Gradient Centrifugation of Erythrocytes-Explanation of Cellular Bands and Compromised Age Separation.
Percoll
age separation
aggregation
band 4.1 protein
band formation
blood sedimentation
complex fluids
density gradient
micromechanical modeling
red blood cells
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
11 04 2022
11 04 2022
Historique:
received:
01
03
2022
revised:
06
04
2022
accepted:
07
04
2022
entrez:
23
4
2022
pubmed:
24
4
2022
medline:
27
4
2022
Statut:
epublish
Résumé
(1) Background: When red blood cells are centrifuged in a continuous Percoll-based density gradient, they form discrete bands. While this is a popular approach for red blood cell age separation, the mechanisms involved in banding were unknown. (2) Methods: Percoll centrifugations of red blood cells were performed under various experimental conditions and the resulting distributions analyzed. The age of the red blood cells was measured by determining the protein band 4.1a to 4.1b ratio based on western blots. Red blood cell aggregates, so-called
Identifiants
pubmed: 35455975
pii: cells11081296
doi: 10.3390/cells11081296
pmc: PMC9028966
pii:
doi:
Substances chimiques
Percoll
65455-52-9
Silicon Dioxide
7631-86-9
Povidone
FZ989GH94E
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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