CinA mediates multidrug tolerance in Mycobacterium tuberculosis.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
22 04 2022
Historique:
received: 01 12 2020
accepted: 31 03 2022
entrez: 23 4 2022
pubmed: 24 4 2022
medline: 27 4 2022
Statut: epublish

Résumé

The ability of Mycobacterium tuberculosis (Mtb) to resist and tolerate antibiotics complicates the development of improved tuberculosis (TB) chemotherapies. Here we define the Mtb protein CinA as a major determinant of drug tolerance and as a potential target to shorten TB chemotherapy. By reducing the fraction of drug-tolerant persisters, genetic inactivation of cinA accelerated killing of Mtb by four antibiotics in clinical use: isoniazid, ethionamide, delamanid and pretomanid. Mtb ΔcinA was killed rapidly in conditions known to impede the efficacy of isoniazid, such as during nutrient starvation, during persistence in a caseum mimetic, in activated macrophages and during chronic mouse infection. Deletion of CinA also increased in vivo killing of Mtb by BPaL, a combination of pretomanid, bedaquiline and linezolid that is used to treat highly drug-resistant TB. Genetic and drug metabolism studies suggest that CinA mediates drug tolerance via cleavage of NAD-drug adducts.

Identifiants

pubmed: 35459278
doi: 10.1038/s41467-022-29832-1
pii: 10.1038/s41467-022-29832-1
pmc: PMC9033802
doi:

Substances chimiques

Antitubercular Agents 0
Isoniazid V83O1VOZ8L

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

2203

Subventions

Organisme : NIAID NIH HHS
ID : R25 AI140472
Pays : United States

Informations de copyright

© 2022. The Author(s).

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Auteurs

Kaj M Kreutzfeldt (KM)

Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY, 10065, USA.

Robert S Jansen (RS)

Division of Infectious Diseases, Department of Medicine, Weill Cornell Medical College, New York, NY, 10065, USA.
Department of Microbiology, Radboud University, 6525 AJ, Nijmegen, The Netherlands.

Travis E Hartman (TE)

Division of Infectious Diseases, Department of Medicine, Weill Cornell Medical College, New York, NY, 10065, USA.

Alexandre Gouzy (A)

Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY, 10065, USA.

Ruojun Wang (R)

Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY, 10065, USA.
Department of Molecular Biology, Princeton University, Princeton, NJ, 08540, USA.

Inna V Krieger (IV)

Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, 77843, USA.

Matthew D Zimmerman (MD)

Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, 07110, USA.

Martin Gengenbacher (M)

Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, 07110, USA.

Jansy P Sarathy (JP)

Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, 07110, USA.

Min Xie (M)

Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, 07110, USA.

Véronique Dartois (V)

Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ, 07110, USA.

James C Sacchettini (JC)

Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, 77843, USA.

Kyu Y Rhee (KY)

Division of Infectious Diseases, Department of Medicine, Weill Cornell Medical College, New York, NY, 10065, USA.
Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY, 10065, USA.

Dirk Schnappinger (D)

Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY, 10065, USA. dis2003@med.cornell.edu.

Sabine Ehrt (S)

Department of Microbiology and Immunology, Weill Cornell Medical College, New York, NY, 10065, USA. sae2004@med.cornell.edu.

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