Visualisation of Phosphate in Subcalicoblastic Extracellular Calcifying Medium and on a Skeleton of Coral by Using a Novel Probe, Fluorescein-4-Isothiocyanate-Labelled Alendronic Acid.
CaCO3
Coral calcification
Fluorescence probe
Phosphate
Journal
Marine biotechnology (New York, N.Y.)
ISSN: 1436-2236
Titre abrégé: Mar Biotechnol (NY)
Pays: United States
ID NLM: 100892712
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
18
01
2022
accepted:
11
03
2022
pubmed:
24
4
2022
medline:
29
6
2022
entrez:
23
4
2022
Statut:
ppublish
Résumé
The overload of nutrients of anthropogenic origin, including phosphate, onto coastal waters has been reported to have detrimental effects on corals. However, to the best of our knowledge, the phosphate concentration threshold for inhibiting coral calcification is unclear owing to a lack of information on the molecular mechanisms involved in the inhibitory effect of phosphate. Therefore, in this study, we prepared a new phosphate analogue, fluorescein-4-isothiocyanate (FITC)-labelled alendronic acid (FITC-AA), from commercially available reagents and used it as a novel probe to demonstrate its transfer pathway from ambient seawater into Acropora digitifera. When the juveniles at 1 d post-settlement were treated with FITC-AA in a laboratory tank, this phosphate analogue was found in the subcalicoblastic extracellular calcifying medium (SCM) and was absorbed on the basal plate in the juveniles within a few minutes. When the juveniles bear zooxanthellae at 3 months post-settlement, FITC-AA was observed on the corallite walls within a few minutes after adding ambient seawater. We concluded that FITC-AA in ambient seawater was transferred via a paracellular pathway to SCM and then absorbed on the coral CaCO
Identifiants
pubmed: 35460469
doi: 10.1007/s10126-022-10115-1
pii: 10.1007/s10126-022-10115-1
doi:
Substances chimiques
Phosphates
0
Fluorescein-5-isothiocyanate
I223NX31W9
Fluorescein
TPY09G7XIR
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
524-530Subventions
Organisme : Environmental Restoration and Conservation Agency
ID : JPMEERF20194007
Organisme : Japan Society for the Promotion of Science
ID : 19K12310
Organisme : Japan Society for the Promotion of Science
ID : 20H03077
Organisme : Japan Society for the Promotion of Science
ID : 21J01671
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
Références
Aihara Y, Maruyama S, Baird AH, Iguchi A, Takahashi S, Minagawa J (2019) Green fluorescence from cnidarian hosts attract symbiotic algae. Proc Natl Acad Sci U S A 116:2118–2123.
doi: 10.1073/pnas.1812257116
pubmed: 30670646
pmcid: 6369807
Allemand D, Tambutté É, Zoccola D, Tambutté S (2011) Coral calcification, cells to reefs. In: Dubinsky Z, Stambler N (eds) Coral reefs: an ecosystem in transition. Springer, Dordrecht, p 119–150, Coral Calcification, Cells to Reefs
Bell PR, Lapointe BE, Elmetri I (2007) Reevaluation of ENCORE: support for the eutrophication threshold model for coral reefs. AMBIO J Hum Environ 36:416–424.
Burke L, Reytar K, Spalding M, Perry A (2011) In reefs at risk revisited. World Resources Institute, Washington, DC
Demes KW, Bell SS, Dawes CJ (2009) The effects of phosphate on the biomineralisation of the green alga, Halimeda incrassata (Ellis) lam. J Exp Mar Biol Ecol 374:123–127.
Dunn JG, Sammarco PW, LaFleur G (2012) Effects of phosphate on growth and skeletal density in the scleractinian coral Acropora muricata: a controlled experimental approach. J Exp Mar Biol Ecol 411:34–44.
Houk P, Comeros-Raynal M, Lawrence A, Sudek M, Vaeoso M, McGuire K, Regis J (2020) Nutrient thresholds to protect water quality and coral reefs. Mar Pollut Bull 159:111451.
Iijima M, Yasumoto J, Iguchi A, Koiso K, Ushigome S, Nakajima N, Kunieda Y, Nakamura T, Sakai K, Yasumoto-Hirose M, Mori-Yasumoto K, Mizusawa N, Amano H, Suzuki A, Jimbo M, Watabe S, Yasumoto K (2021) Phosphate bound to calcareous sediments hampers skeletal development of juvenile coral. R Soc Open Sci 8:201214.
Iijima M, Yasumoto K, Yasumoto J, Yasumoto-Hirose M, Kuniya N, Takeuchi R, Nozaki M, Nanba N, Nakamura T, Jimbo M, Watabe S (2019) Phosphate enrichment hampers the development of juvenile Acropora digitifera coral by inhibiting skeleton formation. Mar Biotechnol (NY) 21:291–300.
Iwao K, Fujisawa T, Hatta M (2002) A cnidarian neuropeptide of the GLWamide family induces metamorphosis of reef-building corals in the genus Acropora. Coral Reefs 21:127–129.
Koop K, Booth D, Broadbent A, Brodie J, Bucher D, Capone D, Coll J, Dennison W, Erdmann M, Harrison P, Hoegh-Guldberg O, Hutchings P, Jones GB, Larkum AW, O’Neil J, Steven A, Tentori E, Ward S, Williamson J, Yellowlees D (2001) ENCORE: the effect of nutrient enrichment on coral reefs. Synthesis of results and conclusions. Mar Pollut Bull 42:91–120.
Le Goff C, Tambutté E, Venn AA, Techer N, Allemand D, Tambutté S (2017) In vivo pH measurement at the site of calcification in an octocoral. Sci Rep 7:11210.
Millero F, Huang F, Zhu X, Liu X, Zhang JZ (2001) Adsorption and desorption of phosphate on calcite and aragonite in seawater. Aquat Geochem 7:33–56.
Norat-Ramírez J, Méndez-Lázaro P, Hernández-Delgado EA, Mattei-Torres H, Cordero-Rivera L (2019) A septic waste index model to measure the impact of septic tanks on coastal water quality and coral reef communities in Rincon, Puerto Rico. Ocean Coast Manag 169:201–213.
Ohno Y, Iguchi A, Shinzato C, Gushi M, Inoue M, Suzuki A, Sakai K, Nakamura T (2017a) Calcification process dynamics in coral primary polyps as observed using a calcein incubation method. Biochem Biophys Rep 9:289–294.
Ohno Y, Iguchi A, Shinzato C, Inoue M, Suzuki A, Sakai K, Nakamura T (2017b) An aposymbiotic primary coral polyp counteracts acidification by active pH regulation. Sci Rep 7:40324.
Rott E, Steinmetz H, Metzger JW (2018) Organophosphonates: a review on environmental relevance, biodegradability and removal in wastewater treatment plants. Sci Total Environ 615:1176–1191.
Sawada K, Abdel-Aal N, Sekino H, Satoh K (2003) Adsorption of inorganic phosphates and organic polyphosphonate on calcite. Dalton Trans 3:342–347.
Suzuki T, Inomata S, Sawada K (1986) Adsorption of phosphate on calcite. J Chem Soc Faraday Trans 1:1733–1743.
Tambutté E, Tambutté S, Segonds N, Zoccola D, Venn A, Erez J, Allemand D (2012) Calcein labelling and electrophysiology: insights on coral tissue permeability and calcification. Proc Biol Sci 279:19–27.
Tisthammer KH, Timmins-Schiffman E, Seneca FO, Nunn BL, Richmond RH (2021) Physiological and molecular responses of lobe coral indicate nearshore adaptations to anthropogenic stressors. Sci Rep 11:3423.
Weston SA, Parish CR (1992) Calcein: a novel marker for lymphocytes which enter lymph nodes. Cytometry:J. Int Soc Anal Cytol 13:739–749.
Wiedenmann J, D’Angelo C, Smith EG, Hunt AN, Legiret FE, Postle AD, Achterberg EP (2013) Nutrient enrichment can increase the susceptibility of reef corals to bleaching. Nature Clim Change 3:160–164.
Wolff NH, Mumby PJ, Devlin M, Anthony KRN (2018) Vulnerability of the Great Barrier Reef to climate change and local pressures. Glob Chang Biol 24:1978–1991.
Yamashiro H (1995) The effects of HEBP, an inhibitor of mineral deposition, upon photosynthesis and calcification in the scleractinian coral, Stylophora pistillata. J Exp Mar Biol Ecol 191:57–63.