A Novel ex vivo Method for Investigating Vascularization of Transplanted Islets.
Angiogenesis
Biomimetic
Islet
Pericyte
Revascularization
Journal
Journal of vascular research
ISSN: 1423-0135
Titre abrégé: J Vasc Res
Pays: Switzerland
ID NLM: 9206092
Informations de publication
Date de publication:
2022
2022
Historique:
received:
14
06
2021
accepted:
01
03
2022
pubmed:
25
4
2022
medline:
22
7
2022
entrez:
24
4
2022
Statut:
ppublish
Résumé
Revascularization of transplanted pancreatic islets is critical for survival and treatment of type 1 diabetes. Questions concerning how islets influence local microvascular networks and how networks form connections with islets remain understudied and motivate the need for new models that mimic the complexity of real tissue. Recently, our laboratory established the rat mesentery culture model as a tool to investigate cell dynamics involved in microvascular growth. An advantage is the ability to observe blood vessels, lymphatics, and immune cells. The objective of this study was to establish the rat mesentery tissue culture model as a useful tool to investigate islet tissue integration. DiI-labeled islets were seeded onto adult rat mesentery tissues and cultured for up to 3 days. Live lectin labeling enabled time-lapse observation of vessel growth. During culture, DiI-positive islets remained intact. Radial lectin-positive capillary sprouts with DiI labeling were observed to form from islets and connect to host networks. Lectin-positive vessels from host networks were also seen growing toward islets. PECAM and NG2 labeling confirmed that vessels sprouting from islets contained endothelial cells and pericytes. Our results introduce the rat mesentery culture model as a platform for investigating dynamics associated with the initial revascularization of transplanted islets.
Identifiants
pubmed: 35462373
pii: 000523925
doi: 10.1159/000523925
pmc: PMC9308658
mid: NIHMS1788808
doi:
Substances chimiques
Lectins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
229-238Subventions
Organisme : NIA NIH HHS
ID : R01 AG049821
Pays : United States
Informations de copyright
© 2022 S. Karger AG, Basel.
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