Capillary Electrophoresis Mass Spectrometry for Scalable Single-Cell Proteomics.
capillary elechophoresis
mass spectrometry
mouse
proteomics
single cell
xenopus
Journal
Frontiers in chemistry
ISSN: 2296-2646
Titre abrégé: Front Chem
Pays: Switzerland
ID NLM: 101627988
Informations de publication
Date de publication:
2022
2022
Historique:
received:
27
01
2022
accepted:
28
02
2022
entrez:
25
4
2022
pubmed:
26
4
2022
medline:
26
4
2022
Statut:
epublish
Résumé
Understanding the biochemistry of the cell requires measurement of all the molecules it produces. Single-cell proteomics recently became possible through advances in microanalytical sample preparation, separation by nano-flow liquid chromatography (nanoLC) and capillary electrophoresis (CE), and detection using electrospray ionization (ESI) high-resolution mass spectrometry (HRMS). Here, we demonstrate capillary microsampling CE-ESI-HRMS to be scalable to proteomics across broad cellular dimensions. This study established proof-of-principle using giant, ∼250-µm-diameter cells from embryos of the frog
Identifiants
pubmed: 35464213
doi: 10.3389/fchem.2022.863979
pii: 863979
pmc: PMC9024316
doi:
Types de publication
Journal Article
Langues
eng
Pagination
863979Informations de copyright
Copyright © 2022 Shen, Pade, Choi, Muñoz-LLancao, Manzini and Nemes.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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