A single intranasal dose of human parainfluenza virus type 3-vectored vaccine induces effective antibody and memory T cell response in the lungs and protects hamsters against SARS-CoV-2.
Journal
NPJ vaccines
ISSN: 2059-0105
Titre abrégé: NPJ Vaccines
Pays: England
ID NLM: 101699863
Informations de publication
Date de publication:
25 Apr 2022
25 Apr 2022
Historique:
received:
13
12
2021
accepted:
21
03
2022
entrez:
26
4
2022
pubmed:
27
4
2022
medline:
27
4
2022
Statut:
epublish
Résumé
Respiratory tract vaccination has an advantage of needle-free delivery and induction of mucosal immune response in the portal of SARS-CoV-2 entry. We utilized human parainfluenza virus type 3 vector to generate constructs expressing the full spike (S) protein of SARS-CoV-2, its S1 subunit, or the receptor-binding domain, and tested them in hamsters as single-dose intranasal vaccines. The construct bearing full-length S induced high titers of neutralizing antibodies specific to S protein domains critical to the protein functions. Robust memory T cell responses in the lungs were also induced, which represent an additional barrier to infection and should be less sensitive than the antibody responses to mutations present in SARS-CoV-2 variants. Following SARS-CoV-2 challenge, animals were protected from the disease and detectable viral replication. Vaccination prevented induction of gene pathways associated with inflammation. These results indicate advantages of respiratory vaccination against COVID-19 and inform the design of mucosal SARS-CoV-2 vaccines.
Identifiants
pubmed: 35468973
doi: 10.1038/s41541-022-00471-3
pii: 10.1038/s41541-022-00471-3
pmc: PMC9038905
doi:
Types de publication
Journal Article
Langues
eng
Pagination
47Subventions
Organisme : University of Texas Medical Branch at Galveston | Institute for Translational Sciences, University of Texas Medical Branch (Institute for Translational Sciences at UTMB)
ID : N/A
Informations de copyright
© 2022. The Author(s).
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