Integrated continuous biomanufacturing on pilot scale for acid-sensitive monoclonal antibodies.
Chinese Hamster Ovary cells
ZCa ligand
antibody aggregation
antibody manufacturing
continuous chromatography
integrated continuous bioprocess
perfusion culture
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
revised:
21
04
2022
received:
19
01
2022
accepted:
23
04
2022
pubmed:
27
4
2022
medline:
14
7
2022
entrez:
26
4
2022
Statut:
ppublish
Résumé
In this study, we demonstrated the first, to our knowledge, integrated continuous bioprocess (ICB) designed for the production of acid-sensitive monoclonal antibodies, prone to aggregate at low pH, on pilot scale. A high cell density perfusion culture, stably maintained at 100 × 10
Identifiants
pubmed: 35470430
doi: 10.1002/bit.28120
pmc: PMC9541590
doi:
Substances chimiques
Antibodies, Monoclonal
0
Staphylococcal Protein A
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2152-2166Informations de copyright
© 2022 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC.
Références
Biotechnol J. 2019 Aug;14(8):e1800646
pubmed: 30810288
Biotechnol Bioeng. 2021 Sep;118(9):3287-3301
pubmed: 33410159
Anal Chem. 2014 May 20;86(10):5150-7
pubmed: 24749737
Biotechnol Bioeng. 2020 Apr;117(4):1024-1036
pubmed: 31930482
Biotechnol J. 2019 Feb;14(2):e1800061
pubmed: 29729129
Biotechnol Bioeng. 2018 Aug;115(8):1949-1957
pubmed: 29663323
MAbs. 2016 Aug-Sep;8(6):1107-17
pubmed: 27310175
J Biotechnol. 2015 Nov 10;213:120-30
pubmed: 26091773
Biotechnol Prog. 2020 Jul;36(4):e2995
pubmed: 32233078
Metab Eng. 2021 May;65:135-145
pubmed: 33161144
J Mol Biol. 2018 Sep 14;430(18 Pt B):3427-3438
pubmed: 29886013
J Biotechnol. 2015 Nov 10;213:3-12
pubmed: 26014522
Biotechnol Bioeng. 2021 Apr;118(4):1664-1676
pubmed: 33459355
J Chromatogr A. 2012 Jan 20;1221:57-70
pubmed: 22071423
Biotechnol Bioeng. 2021 Sep;118(9):3313-3322
pubmed: 33480439
J Biotechnol. 2015 Nov 10;213:28-41
pubmed: 26211737
Biotechnol Bioeng. 2021 Sep;118(9):3533-3544
pubmed: 33914903
Biotechnol Bioeng. 2020 May;117(5):1367-1380
pubmed: 32022243
J Chromatogr A. 2009 Oct 9;1216(41):6938-45
pubmed: 19733359
MAbs. 2019 Nov-Dec;11(8):1492-1501
pubmed: 31526164
Protein Sci. 2013 Nov;22(11):1542-51
pubmed: 23963869
Protein Expr Purif. 2017 Jun;134:96-103
pubmed: 28414067
Transfusion. 2009 Sep;49(9):1931-43
pubmed: 19497061
Biotechnol Prog. 2017 Jul;33(4):923-930
pubmed: 28054475
J Chromatogr A. 2021 Jan 4;1635:461760
pubmed: 33271430
J Chromatogr A. 2007 Nov 9;1171(1-2):22-8
pubmed: 17920607
Prep Biochem Biotechnol. 2018 May 28;48(5):383-390
pubmed: 29509101
Biotechnol J. 2016 Jan;11(1):135-45
pubmed: 26308369
Biotechnol Prog. 2013 May-Jun;29(3):754-67
pubmed: 23436789
Biotechnol Prog. 2017 Jul;33(4):954-965
pubmed: 28188705
Biotechnol Bioeng. 2012 Dec;109(12):3018-29
pubmed: 22729761
J Chromatogr A. 2020 Jun 21;1621:461055
pubmed: 32276855
Biotechnol Prog. 2013 May-Jun;29(3):768-77
pubmed: 23436783
J Biotechnol. 2015 Nov 10;213:13-9
pubmed: 26073998
Biotechnol Prog. 2017 Jul;33(4):854-866
pubmed: 28480535
Biochemistry. 2010 Nov 2;49(43):9328-38
pubmed: 20843079
Biotechnol Bioeng. 2021 Apr;118(4):1721-1735
pubmed: 33491769
Biotechnol Prog. 2017 Sep;33(5):1303-1313
pubmed: 28691347
J Biotechnol. 2020 Feb 10;309:44-52
pubmed: 31891733
BMC Biotechnol. 2014 Nov 29;14:99
pubmed: 25431119
Biotechnol Bioeng. 2011 Jul;108(7):1494-508
pubmed: 21480193
J Chromatogr A. 2022 Feb 8;1664:462806
pubmed: 35033788
Cell Mol Immunol. 2020 May;17(5):451-461
pubmed: 32313210
J Biotechnol. 2019 Aug 10;301:45-51
pubmed: 31145936
Biotechnol J. 2012 Dec;7(12):1496-508
pubmed: 23070975
J Chromatogr A. 2021 Jan 25;1637:461843
pubmed: 33412291
Biotechnol Bioeng. 2022 Aug;119(8):2152-2166
pubmed: 35470430