Functional analysis of nuclear receptor HR96 gene in Bombyx mori exposed to phoxim.
Bombyx mori
HR96
nuclear receptor
phoxim
Journal
Archives of insect biochemistry and physiology
ISSN: 1520-6327
Titre abrégé: Arch Insect Biochem Physiol
Pays: United States
ID NLM: 8501752
Informations de publication
Date de publication:
Oct 2022
Oct 2022
Historique:
revised:
23
03
2022
received:
17
02
2022
accepted:
06
04
2022
pubmed:
27
4
2022
medline:
17
9
2022
entrez:
26
4
2022
Statut:
ppublish
Résumé
The nuclear receptor (NRs) gene family functions as ligand-dependent transcription factors in a variety of animals, which participates in a variety of biological processes, such as cell differentiation, metabolic regulation, reproduction, development, insect metamorphosis. In this study, a nuclear receptor HR96 gene in silkworm Bombyx mori (BmHR96) was identified, and the responses of BmHR96 gene to 20-hydroxyecdysone (20E), three insecticides, and two disinfectants were analyzed and its function in phoxim exposure was explored. Quantitative real-time polymerase chain reaction indicated that the expression of BmHR96 mRNA was the highest in ovary of 5th instar Day 3 silkworm larvae and in silk gland of the wandering stage. The expression patterns of BmHR96 gene in ovary, head, testis, and midgut of different stages were different. After injecting 20E into B. mori, the expression of BmHR96 mRNA had no significant difference compared with control. Three insecticides and two disinfectants were used to treat B. mori, respectively, and it was found that they had different influence patterns on the expression level of BmHR96. siRNA of BmHR96 was injected into silkworm larvae and the expression of BmHR96 was decreased significantly after injecting 72 h. After silencing of BmHR96, B. mori was fed with phoxim-treated leaves. The results showed that the mortality of B. mori after silencing of BmHR96 was significantly higher than the control. Our results indicated that HR96 plays an important role in regulating the stress response of phoxim.
Substances chimiques
Disinfectants
0
Insect Proteins
0
Insecticides
0
Organothiophosphorus Compounds
0
RNA, Messenger
0
phoxim
6F5V775VPO
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e21910Subventions
Organisme : National Innovation and Entrepreneurship Project for College students
ID : 202110364065
Organisme : Graduate Student Innovation Fund of Anhui Agricultural University
ID : 2021yjs-39
Organisme : Innovative Research Groups of Anhui Agricultural University
ID : ANRC2019032
Informations de copyright
© 2022 Wiley Periodicals LLC.
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