A transcriptomic atlas of
Aedes
Anopheles
RNAseq atlas
aedes aegypti
anopheles gambiae
blood feeding
chromosomes
gene expression
gut transcriptome
infectious disease
innate immunity
microbiology
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
26 04 2022
26 04 2022
Historique:
received:
06
12
2021
accepted:
25
04
2022
pubmed:
27
4
2022
medline:
20
5
2022
entrez:
26
4
2022
Statut:
epublish
Résumé
Mosquitoes transmit numerous pathogens, but large gaps remain in our understanding of their physiology. To facilitate explorations of mosquito biology, we have created Aegypti-Atlas (http://aegyptiatlas.buchonlab.com/), an online resource hosting RNAseq profiles of Ae. aegypti body parts (head, thorax, abdomen, gut, Malpighian tubules, ovaries), gut regions (crop, proventriculus, anterior and posterior midgut, hindgut), and a gut time course of blood meal digestion. Using Aegypti-Atlas, we provide insights into regionalization of gut function, blood feeding response, and immune defenses. We find that the anterior and posterior midgut possess digestive specializations which are preserved in the blood-fed state. Blood feeding initiates the sequential induction and repression/depletion of multiple cohorts of peptidases. With respect to defense, immune signaling components, but not recognition or effector molecules, show enrichment in ovaries. Basal expression of antimicrobial peptides is dominated by holotricin and gambicin, which are expressed in carcass and digestive tissues, respectively, in a mutually exclusive manner. In the midgut, gambicin and other effectors are almost exclusively expressed in the anterior regions, while the posterior midgut exhibits hallmarks of immune tolerance. Finally, in a cross-species comparison between Ae. aegypti and Anopheles gambiae midguts, we observe that regional digestive and immune specializations are conserved, indicating that our dataset may be broadly relevant to multiple mosquito species. We demonstrate that the expression of orthologous genes is highly correlated, with the exception of a 'species signature' comprising a few highly/disparately expressed genes. With this work, we show the potential of Aegypti-Atlas to unlock a more complete understanding of mosquito biology.
Identifiants
pubmed: 35471187
doi: 10.7554/eLife.76132
pii: 76132
pmc: PMC9113746
doi:
pii:
Substances chimiques
Sugars
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIAID NIH HHS
ID : R01 AI148529
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI148541
Pays : United States
Organisme : NIA NIH HHS
ID : R21 AG065733
Pays : United States
Informations de copyright
© 2022, Hixson et al.
Déclaration de conflit d'intérêts
BH, XB, XY, AB, PN, NB No competing interests declared
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