Fluorescent bioassay for SARS-CoV-2 detection using polypyrene-g-poly(ε-caprolactone) prepared by simultaneous photoinduced step-growth and ring-opening polymerizations.
Fluorescent biosensor
Graft copolymer
Photopolymerization
Ring-opening polymerization
SARS-CoV-2
Step-growth polymerization
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
26 04 2022
26 04 2022
Historique:
received:
11
01
2022
accepted:
22
02
2022
entrez:
27
4
2022
pubmed:
28
4
2022
medline:
29
4
2022
Statut:
epublish
Résumé
The construction of a rapid and easy immunofluorescence bioassay for SARS-CoV-2 detection is described. We report for the first time a novel one-pot synthetic approach for simultaneous photoinduced step-growth polymerization of pyrene (Py) and ring-opening polymerization of ε-caprolactone (PCL) to produce a graft fluorescent copolymer PPy-g-PCL that was conjugated to SARS-CoV-2-specific antibodies using EDC/NHS chemistry. The synthesis steps and conjugation products were fully characterized using standard spectral analysis. Next, the PPy-g-PCL was used for the construction of a dot-blot assay which was calibrated for applications to human nasopharyngeal samples. The analytical features of the proposed sensor showed a detection range of 6.03-8.7 LOG viral copy mL
Identifiants
pubmed: 35474492
doi: 10.1007/s00604-022-05244-2
pii: 10.1007/s00604-022-05244-2
pmc: PMC9042169
doi:
Substances chimiques
Caproates
0
Coloring Agents
0
Lactones
0
Polyesters
0
Poly A
24937-83-5
polycaprolactone
24980-41-4
caprolactone
56RE988L1R
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
202Subventions
Organisme : The Scientific and Technological Research Council of Turkey
ID : 120C121
Organisme : Bilimsel Araştirma Projeleri Birimi, Istanbul Üniversitesi
ID : TUB-2021-43195
Organisme : Ege Üniversitesi
ID : TOA-2020-21862
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.
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